Western Blot: Cre Antibody [NB100-56133] - Analysis of Cre using NB100-56133 at 1:2000. Lane 1: HeLa cells stably transfected with a CRE expression plasmid. Lane 2: HeLa cells transfected with a neo plasmid (negative ...read more
Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133] - Representative co-IF image showing YFP (green) and Cre (red) staining on a MG section from a Trp53L/L;R26Y female 3 days after Ad-K8-Cre injection. ...read more
Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the ...read more
Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the neurons brain. This mouse ...read more
Immunohistochemistry: Cre Antibody [NB100-56133] - Enkephalin expression in the Paraventricular nucleus of the hypothalamus (PVN) in mouse brain. Antibody at 1:500. Image from verified customer review.
Specific to Cre/lox system (Bacteriophage). Shown to detect Cre protein in Cre-transfected human cells (HeLa) and Cre-transgenic mice. Mouse reactivity reported in scientific literature (PMID: 28194015).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for Cre Antibody - BSA Free
Cyclization recombinase
Background
Cre recombinase or cyclization recombination enzyme is a bacteriophage-P1 enzyme belonging to large family of recombinases which are required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state. Cre is well known for facilitating a site-specific recombination between two 34 base pair loxP sites. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule, and the excised DNA segment is subsequently degraded. Cre and lox comprises Cre/lox system which has been used extensively for multiple in vitro and in vivo applications including targeted gene deletions, gene-specific humanized animal models, as well as in plants and microorganisms stocks.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Cre Antibody (NB100-56133)(13)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 6 applications: Flow Cytometry, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.
FAQs for Cre Antibody (NB100-56133). (Showing 1 - 2 of 2 FAQs).
A customer is looking for a Cre Antibody, raised in either rabbit or goat, suitable for IHC-F. Would you know of a suitable Cre antibody that fits the above criteria and also been published for IHC-Fr? If known, can you also provide details of the publication?
With regards to your customer's inquiry on our Cre antibodies, we do have a wide range of antibodies that would be suitable to use in IHC for frozen sections. NB100-56134 for instance has been tested and validated for IHC-fry and there is one cited PubMed publication on it as well.
Can I use this antibody for protein immunoblots of flox/flox and flox/flox cre animals to show cre is expressed only in the flox/flox cre animals?
Our NB100-56133 Cre antibody is specific to the cre enzyme. When using this antibody, you should only detect a band on your blot when the cre enzyme is present. There should be no detection of your floxed mice as long as cre is not expressed or added.
Understanding DNA Recombination with Cre-Lox Cyclization recombination enzyme (Cre) is a member of the extensive family of recombinases and recognizes a 34 bp sequence motif from PI bacteriophage referred to as LoxP. The Cre enzyme works to cleanly excise an intervening DNA fragment that is flan... Read full blog post.
Cre/Lox: The Genomic Utility Knife Cre (Cyclization recombination enzyme) is a member of the large family of recombinases. Cre recognizes Lox site loxP, a 34 bp sequence motif from the PI bateriophage. If a DNA segment is flanked by two loxP sites in the same orientation, Cre neatly ex... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Cre Antibody [NB100-56133] - Analysis of Cre using NB100-56133 at 1:2000. Lane 1: HeLa cells stably transfected with a CRE expression plasmid. Lane 2: HeLa cells transfected with a neo plasmid (negative control).](http://images.novusbio.com/fullsize/Cre-Antibody-Western-Blot-NB100-56133-img0004.jpg "Western Blot: Cre Antibody [NB100-56133] - Analysis of Cre using NB100-56133 at 1:2000. Lane 1: HeLa cells stably transfected with a CRE expression plasmid. Lane 2: HeLa cells transfected with a neo plasmid (negative control).")
![Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133] - Representative co-IF image showing YFP (green) and Cre (red) staining on a MG section from a Trp53L/L;R26Y female 3 days after Ad-K8-Cre injection. Trp53L/L;R26Y females (n=3); R26Y females (n=3). Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28194015/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cre-Antibody---BSA-Free-Immunohistochemistry-NB100-56133-img0008.jpg "Immunohistochemistry: Cre Antibody - BSA Free [NB100-56133] - Representative co-IF image showing YFP (green) and Cre (red) staining on a MG section from a Trp53L/L;R26Y female 3 days after Ad-K8-Cre injection. Trp53L/L;R26Y females (n=3); R26Y females (n=3). Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28194015/) licensed under a CC-BY license.")
![Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56133-img0005.jpg "Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.")
![Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56133-img0006.jpg "Immunohistochemistry: Cre Antibody [NB100-56133] - FFPE tissue section from the cerebellum of a Cre transgenic mouse using NB100-56133 at 1:2000. In this mouse line, Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.")
![Immunohistochemistry: Cre Antibody [NB100-56133] - Enkephalin expression in the Paraventricular nucleus of the hypothalamus (PVN) in mouse brain. Antibody at 1:500. Image from verified customer review.](http://images.novusbio.com/fullsize/Cre-Antibody-Immunohistochemistry-NB100-56133-img0007.jpg "Immunohistochemistry: Cre Antibody [NB100-56133] - Enkephalin expression in the Paraventricular nucleus of the hypothalamus (PVN) in mouse brain. Antibody at 1:500. Image from verified customer review.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
