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CPS1 Antibody

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Western Blot: CPS1 Antibody [NBP1-54775] - Titration: 5.0ug/ml Positive Control: Human Fetal liver cell lysate.
Immunohistochemistry: CPS1 Antibody [NBP1-54775] - Pig ileum Primary Antibody Dilution: 1 : 500 Secondary Antibody: Anti-rabbit-biotin, streptavidin-HRP Secondary Antibody Dilution: 1 : 500Color/Signal Descriptions: ...read more
Immunohistochemistry-Paraffin: CPS1 Antibody [NBP1-54775] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X ...read more
Immunohistochemistry: CPS1 Antibody [NBP1-54775] - Pig duodenum Primary Antibody Dilution: 1 : 500 Secondary Antibody: Anti-rabbit-biotin, streptavidin-HRP Secondary Antibody Dilution: 1 : 500Color/Signal Descriptions: ...read more
Immunohistochemistry: CPS1 Antibody [NBP1-54775] - Pig kidney Primary Antibody Dilution: 1 : 500 Secondary Antibody: Anti-rabbit-biotin, streptavidin-HRP Secondary Antibody Dilution: 1 : 500. Color/Signal Descriptions: ...read more

Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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CPS1 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to CPS1(carbamoyl-phosphate synthetase 1, mitochondrial) The peptide sequence was selected from the middle region of CPS1. Peptide sequence YPSVTNYLYVTYNGQEHDVNFDDHGMMVLGCGPYHIGSSVEFDWCAVSSI. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPS1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1.0 ug/ml
Theoretical MW
165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CPS1 Antibody

  • carbamoyl-phosphate synthase [ammonia], mitochondrial
  • carbamoyl-phosphate synthase 1, mitochondrial
  • carbamoyl-phosphate synthetase 1, mitochondrial
  • carbamoylphosphate synthetase I
  • Carbamoyl-phosphate synthetase I
  • CPSase I
  • CPSASE1
  • EC 6.3.4.16

Background

Carbamoyl phosphate synthetase I is the rate-limiting enzyme that catalyzes the first committed step of the hepatic urea cycle. The mitochondrial isozyme is designated CPS I and the cytoplasmic enzyme CPS II. CPS II is part of a multifunctional enzyme, called the CAD trifunctional protein of pyrimidine biosynthesis.Carbamoyl phosphate synthetase I (EC 6.3.4.16) is the rate-limiting enzyme that catalyzes the first committed step of the hepatic urea cycle. The mitochondrial isozyme is designated CPS I and the cytoplasmic enzyme CPS II. CPS II is part of a multifunctional enzyme, called the CAD trifunctional protein of pyrimidine biosynthesis (CAD; MIM 114010), which has been mapped to 2p21.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CPS1
Uniprot