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Connexin 30.1/GJB5 Recombinant Protein Antigen

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

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Connexin 30.1/GJB5 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human GJB5.

Source: E. coli

Amino Acid Sequence: KRCHECLAARKAQAMCTGHHPHGTTSSCKQDDLLSGDLIFLGSDSHPPLLPDRPRDHVKK

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
GJB5
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-84333.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

For further blocking peptide related information and a protocol, click here.

Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Connexin 30.1/GJB5 Recombinant Protein Antigen

  • Connexin 30.1
  • connexin 31.1
  • connexin-31.1
  • CX31.1
  • CX31.1gap junction beta-5 protein
  • gap junction protein, beta 5 (connexin 31.1)
  • gap junction protein, beta 5
  • gap junction protein, beta 5, 31.1kDa
  • GJB5

Background

GJB5, also known as Gap junction beta-5 protein, is a 273 amino acid protein that is 31 kDa. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, that provide direct intracellular communication. Current research is being performed on this proteins involvement in erythrokeratodermia variabilis, peripheral neuropathy, neuropathy, neuronitis, erythrokeratoderma, and hearing loss. This protein has been linked to signaling in Gap junctions pathway and interacting GSK3B protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol GJB5