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Complement C4c Antibody (033D-69.4.1 (10-12))

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
033D-69.4.1 (10-12)
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Complement C4c Antibody (033D-69.4.1 (10-12)) Summary

Immunogen
Purified human C4c
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
C4B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Clone 1012 binds both C4c and C4 and so is not suitable for all ELISA applications.
Control
C4 binding protein A Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Borate buffered saline
Preservative
<0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Complement C4c Antibody (033D-69.4.1 (10-12))

  • basic C4
  • Basic complement C4
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
  • C4B1
  • C4B12
  • C4B2
  • C4B3
  • C4FMGC164979
  • CH
  • Chido form of C4
  • CO4C4B5
  • complement C4-B
  • complement C4B1a
  • complement component 4B (Chido blood group)
  • complement component 4B
  • CPAMD3FLJ60561
  • EC 2.1.1.144
  • EC 2.7.11

Background

C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Complement C4c Antibody (NB100-63261). (Showing 1 - 1 of 1 FAQ).

  1. Regarding the following product, could you let us know the buffer formulation?
    • Disodium tetraborate decahydrate (CAS#1303-96-4) - 3.7586% Sodium Azide (CAS#26628-22-8) - <0.1%

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Array NB100-63261

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Bioinformatics

Gene Symbol C4B
Entrez
Uniprot