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Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric)

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Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric) [NBP3-24479] - Assay Example
Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric) [NBP3-24479] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Animal tissue
Standard Curve Range
1.21 - 40 U/g fresh weight
Sensitivity
1.21 U/g fresh weight

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Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric) Summary

Description
Detection instrument: Microplate reader (324 nm)
Assay time: 65 min
Standard Curve Range
1.21 - 40 U/g fresh weight
Sensitivity
1.21 U/g fresh weight
Inter-Assay
%CV < 9.4
Intra-Assay
%CV < 5.1
Kit Type
Activity Assay Kit (Colorimetric)
Gene
CHAT

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Plate Sealer
  2. Reagent 1: Buffer Solution
  3. Reagent 2: Inhibitor
  4. Reagent 3: Substrate A
  5. Reagent 4: Substrate B
  6. Reagent 5: Accelerant A
  7. Reagent 6: Accelerant B
  8. Reagent 7: Chromogenic Agent
  9. UV Microplate

Alternate Names for Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric)

  • acetyl CoA:choline O-acetyltransferase
  • CHAT
  • CHOACTase
  • Choline acetylase
  • Choline Acetyltransferase
  • choline O-acetyltransferase
  • CMS1A
  • CMS1A2
  • EC 2.3.1
  • EC 2.3.1.6

Background

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Choline Acetyltransferase (ChAT) – a useful Immunohistochemical marker for morphological studies of neurons
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Product PDFs

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  Manual - Choline Acetyltransferase/ChAT Activity Assay Kit (Colorimetric)
  PDF Datasheet
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Bioinformatics

Gene Symbol CHAT