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Rat CENPB ELISA Kit (Colorimetric)

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ELISA: Rat CENPB ELISA Kit (Colorimetric) [NBP2-75227] - Samples were spiked with high concentrations of Rat CENPB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat CENPB ELISA Kit (Colorimetric) [NBP2-75227] - Samples were spiked with high concentrations of Rat CENPB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat CENPB ELISA Kit (Colorimetric) [NBP2-75227] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

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Rat CENPB ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CENPB in samples. No significant cross-reactivity or interference between Rat CENPB and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.17%
Intra-Assay
CV% < 4.81%
Spike Recovery
86-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CENPB

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat CENPB ELISA Kit (Colorimetric)

  • CENP-B
  • centromere autoantigen B
  • centromere protein B (80kD)
  • Centromere protein B
  • centromere protein B, 80kDa
  • major centromere autoantigen B

Background

CENPB product is a highly conserved protein associated with the centromere. It is a DNA-binding protein containing a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Rat CENPB ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol CENPB