| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, CyTOF-ready |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human CELSR2 Cys51-Phe231 Accession # Q9HCU4 |
| Specificity | Detects human CELSR2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR3 is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | CELSR2 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Publications |
|
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2; also cadherin family member 10/CDHF10, Flamingo1 and EGFL2) is a 300-330 kDa member of the LN‑7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51‑231, human CELSR2 shares 93% aa identity with mouse CELSR2.
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry Sortilin Antibody [Unconjugated]](https://images.novusbio.com/images2/Sortilin_AF2934_Immunohistochemistry_7161.jpg)
![Western Blot Sortilin Antibody [Unconjugated]](https://images.novusbio.com/images2/Sortilin_AF2934_Western_Blot_19100.jpg)
![Simple Western Sortilin Antibody [Unconjugated]](https://images.novusbio.com/images2/Sortilin_AF2934_Simple_Western_20098.jpg)
![Frizzled-9 Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Frizzled-9 Antibody [Unconjugated]")
![Immunohistochemistry Frizzled-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Frizzled-3_AF1001_Immunohistochemistry_9912.jpg)
![Western Blot BMP-1/PCP Antibody [Unconjugated]](https://images.novusbio.com/images2/BMP1_AF1927_Western_Blot_21018.jpg)
![Western Blot BMP-1/PCP Antibody [Unconjugated]](https://images.novusbio.com/images2/BMP1_AF1927_Western_Blot_21019.jpg)
![Immunohistochemistry Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]](https://images.novusbio.com/images2/Apolipoprotein_B_MAB4124_Immunohistochemistry_22141.jpg)
![Western Blot Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]](https://images.novusbio.com/images2/Apolipoprotein_B_MAB4124_Western_Blot_20437.jpg)
![Simple Western Apolipoprotein B/ApoB Antibody (369717) [Unconjugated]](https://images.novusbio.com/images/antibody/mab4124_human-apolipoprotein-b-apob-mab-clone-369717-simple-western-301202492438..jpg)
