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CEACAM8/CD66b Antibody [Unconjugated]

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Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM‑8/CD66b Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4246, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM‑8/CD66b Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4246, filled histogram) or isotype control antibody (Catalog # 5-001-A, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF4246
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4246-SP
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CEACAM8/CD66b Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CEACAM‑8/CD66b
Gln35-His141
Accession # P31997
Specificity
Detects human CEACAM‑8/CD66b in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) CEACAM-3, rhCEACAM-6, and rhCEACAM-7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CEACAM8
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF4246 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM8/CD66b Antibody [Unconjugated]

  • Carcinoembryonic antigen CGM6
  • carcinoembryonic antigen gene family member 6
  • carcinoembryonic antigen-related cell adhesion molecule 8
  • CD66b antigen
  • CD66b
  • CD67 antigen
  • CD67
  • CEACAM8
  • CEACAM-8
  • CGM6
  • NCA-95
  • Non-specific cross-reacting antigen NCA-95

Background

CEACAM-8 (carcinoembryonic antigen-related cell adhesion molecule 8; also CD66b, CD67 and NCA-95) is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF4246
CEACAM8/CD66b Antibody [Unconjugated]
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Applications: WB, Flow, CyTOF-ready

Publications for CEACAM8/CD66b Antibody (AF4246)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol CEACAM8
Uniprot