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CDX2 Antibody (963809) [Unconjugated]

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CDX2 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Mouse Anti-Human CDX2 Monoclonal Antibody (Catalog # MAB3665) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
CDX2 was detected in immersion fixed SW480 human colorectal adenocarcinoma cell line using Mouse Anti-Human CDX2 Monoclonal Antibody (Catalog # MAB3665) at 8 µg/mL for 3 hours at room temperature. Cells were stained ...read more
CDX2 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human CDX2 Monoclonal Antibody (Catalog # MAB3665) at 5 µg/mL for 1 hour at room temperature ...read more
BMP7v treatment promotes CR-CSC differentiation. a Phase-contrast microscopy analysis of CD44v6+ CRC sphere cells treated with BMP7v at the indicated time points. One representative of CSC#1, 2, 4, 5, 7, and 23–26 is ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, ICC/IF
Clone
963809
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3665-SP
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CDX2 Antibody (963809) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CDX2
Asn54-Thr185
Accession # Q99626
Specificity
Detects human CDX2 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
Publications
Read Publication using
MAB3665 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDX2 Antibody (963809) [Unconjugated]

  • caudal type homeo box transcription factor 2
  • caudal type homeobox 2
  • Caudal-type homeobox protein 2
  • CDX2
  • CDX3
  • CDX-3
  • CDX3caudal type homeobox transcription factor 2
  • homeobox protein CDX-2

Background

Human CDX2 (Caudal type homeobox transcription factor 2) is a 37 kDa member of the caudal family of homeobox proteins. It is 313 amino acids (aa) in length and contains a 167 aa caudal-like activation domain (aa 13‑180), a DNA-binding homeodomain (aa 186‑245) and two short, poly-glutamine and -proline repeats. CDX2 can exist as a dimer and is regulated by phosphorylation of a 4(x)serine motif that promotes CDX2 degradation. CDX2 promotes intestinal patterning and development. While it is necessary but not sufficient for intestinal phenotype differentiation, it will block cell proliferation on its own. Over the amino acid range used for immunization, human CDX2 is 95% and 97% aa identical to mouse and canine CDX2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDX2 Antibody (MAB3665)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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