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CD81 Antibody (1D6) [Allophycocyanin]

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Product Details

Summary
Reactivity Hu, Mu, Gt, Pm, ShSpecies Glossary
Applications WB, DB, ELISA, Flow, ICC/IF, IHC, IP, CyTOF-ready
Clone
1D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

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CD81 Antibody (1D6) [Allophycocyanin] Summary

Additional Information
Clone 1D6 was used by HLDA to establish CD designation.
Immunogen
Aggregated OCI-Ly8 human B cell line
Localization
Membrane; Multi-pass membrane protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD81
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Dot Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (Ji et al). Canine reactivity reported from a verified customer review. Primate reactivity reported in scientific literature (PMID:33077711).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for CD81 Antibody (1D6) [Allophycocyanin]

  • CD81 antigen (target of antiproliferative 1)
  • CD81 antigen
  • CD81 molecule
  • CD81
  • CVID6
  • TAPA1
  • TAPA-1
  • Target of the antiproliferative 1
  • Tetraspanin-28
  • TSPAN28
  • Tspan-28
  • tspan-28,26 kDa cell surface protein TAPA-1
  • TSPAN28S5.7

Background

CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse. (1) CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma delta + T cells to more mature T cells with alpha beta T cell receptors. (2) Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pT alpha. (2) The monoclonal antibody 2F7 can block thymocyte interaction with CD81 in vitro. (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD81 Antibody (NB100-65805APC) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD81 Antibody (NB100-65805APC) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD81