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Recombinant Mouse CCL27/CTACK Protein

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SDS-Page: Mouse CCL27/CTACK Protein [NBP2-35164]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

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Recombinant Mouse CCL27/CTACK Protein Summary

Description
A single, non-glycosylated polypeptide chain containing 95 amino acids corresponding to CCL27/CTACK Source: E. coli

Uniprot ID: Q9Z1X0

Amino Acid Sequence: LPLPSSTSCC TQLYRQPLPS RLLRRIVHME LQEADGDCHL QAVVLHLARR SVCVHPQNRS LARWLERQGK RLQGTVPSLN LVLQKKMYSN PQQQN

Details of Functionality
CCL27 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 10-100 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CCL27
Purity
> 98 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of CCL27/CTACK as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
10.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, with 5% trehalose.
Preservative
5% Trehalose
Concentration
LYOPH
Purity
> 98 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Mouse CCL27/CTACK Protein

  • ALP
  • C-C motif chemokine 27
  • CCL27
  • chemokine (C-C motif) ligand 27
  • CTACK
  • CTACK/CCL27
  • CTACKSmall-inducible cytokine A27
  • CTAK
  • cutaneous T-cell attracting chemokine
  • Cutaneous T-cell-attracting chemokine
  • ESKINE
  • IL-11 Ralpha-locus chemokine
  • ILC
  • ILCCC chemokine ILC
  • PESKY
  • SCYA27IL-11 R-alpha-locus chemokine
  • skinkine
  • small inducible cytokine subfamily A (Cys-Cys), member 27

Background

CCL27, also named IL-11 R-alpha-locus chemokine (ILC), skinkine, eskine and cutaneous T-cell-attracting chemokine (CTACK), is a small cytokine belonging to the CC chemokine family. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin and it elicits its chemotactic effects by binding to the chemokine receptor CCR10. CCL27 can attract skin-associated memory T-lymphocytes. Studies showed it may play a role in mediating homing of lymphocytes to cutaneous sites and cell migration during embryogenesis. Mature mouse CCL27 is a 95 amino acid (a.a.) protein that shares 57 % a.a. and 87 % a.a. sequence identity with human and rat CCL27, respectively. It shares 18 %~31 % a.a. sequence identity with other mouse CC chemokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CCL27