Recombinant Mouse CCL27/CTACK Protein Summary
Description |
A single, non-glycosylated polypeptide chain containing 95 amino acids corresponding to CCL27/CTACK Source: E. coli Uniprot ID: Q9Z1X0 Amino Acid Sequence: LPLPSSTSCC TQLYRQPLPS RLLRRIVHME LQEADGDCHL QAVVLHLARR SVCVHPQNRS LARWLERQGK RLQGTVPSLN LVLQKKMYSN PQQQN |
Details of Functionality |
CCL27 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 10-100 ng/ml. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
CCL27 |
Purity |
> 98 % pure by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 1 EU/ug of CCL27/CTACK as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
10.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, with 5% trehalose. |
Preservative |
5% Trehalose |
Concentration |
LYOPH |
Purity |
> 98 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Notes
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Mouse CCL27/CTACK Protein
Background
CCL27, also named IL-11 R-alpha-locus chemokine (ILC), skinkine, eskine and cutaneous T-cell-attracting chemokine (CTACK), is a small cytokine belonging to the CC chemokine family. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin and it elicits its chemotactic effects by binding to the chemokine receptor CCR10. CCL27 can attract skin-associated memory T-lymphocytes. Studies showed it may play a role in mediating homing of lymphocytes to cutaneous sites and cell migration during embryogenesis. Mature mouse CCL27 is a 95 amino acid (a.a.) protein that shares 57 % a.a. and 87 % a.a. sequence identity with human and rat CCL27, respectively. It shares 18 %~31 % a.a. sequence identity with other mouse CC chemokines.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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