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Caspase-13 Antibody - BSA Free

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Western Blot: Caspase-13 Antibody [NBP1-76812] - Human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 ug/ml.
Immunohistochemistry: Caspase-13 Antibody - BSA Free [NBP1-76812] - Immunohistochemistry of caspase-13 in mouse brain tissue with caspase-13 antibody at 10 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Caspase-13 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 15 amino acid peptide near the amino terminus of bovine Caspase-13. The immunogen is located within amino acids 80 - 130 of Caspase-13. Amino Acid Squence: ETVAGPDESVGSAAT
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP4
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 ug/ml
Application Notes
A 43 kDa band should be detected in Western Blot.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Staurosporine Treated / Untreated Cell Lysate
Control Peptide
Caspase-13 Protein (NBP1-76812PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Ion exchange chromatography

Alternate Names for Caspase-13 Antibody - BSA Free

  • apoptosis-related cysteine protease
  • CASP13
  • Caspase13
  • Caspase-13
  • ERICE

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death is finally caused by members of the caspase family of proteases and caspase activated DNases. A novel member in the caspase family was recently identified and designated ERICE (for Evolutionarily Related Interleukin-1 beta Converting Enzyme) and caspase-13. Caspase-13 belongs to the ICE subfamily of caspases. Overexpression of caspase-13 induces apoptosis. Caspase-13 was activated by caspase-8, which is a key enzyme in death receptor induced apoptosis. Caspase-13 is expressed in a variety of human tissues and cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CASP4
Uniprot