![Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Mouse liver extract was separated on a 4-15% gel and transfered to PVDF membrane. The membrane was probed with anti-caspase 11 antibody and detected with an anti-mouse HRP secondary antibody. Both procaspase 11 and fully cleaved caspase 11 are detected. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Western-Blot-NBP2-80612-img0003.jpg "Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Mouse liver extract was separated on a 4-15% gel and transfered to PVDF membrane. The membrane was probed with anti-caspase 11 antibody and detected with an anti-mouse HRP secondary antibody. Both procaspase 11 and fully cleaved caspase 11 are detected. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IP, MA, WB, CyTOF-ready, KD, KO |
| Clone | 17D9 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | A recombinant p30 subunit of mouse Caspase 11. [UniProt# P70343] |
| Localization | Cytoplasmic (Endoplasmic reticulum membrane and Mitochondrion) |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | Casp4 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ... Read full blog post. |
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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T... Read full blog post. |
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Caspase 11 - A proinflammatory caspase that induces the innate immune response While known for their role in programmed cell death, caspases are also essential for mediating inflammatory responses and innate immunity. Binding of microbial molecules by pattern recognition receptors triggers the formation of the multiprotein in... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | Casp4 |
![Immunocytochemistry/Immunofluorescence: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Caspase 11 antibody was tested in NIH-3T3 cells at a 1:100 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80612-img0004.jpg "Immunocytochemistry/Immunofluorescence: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Caspase 11 antibody was tested in NIH-3T3 cells at a 1:100 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI. Image from the standard format of this antibody.")
![Immunohistochemistry: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse lung using Caspase 11 antibody (clone 17D9) at 1:100 diluton. The antibody generated cytoplasmic signal with more intense staining in the bronchiolar epithelium while the stainin](http://images.novusbio.com/fullsize/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80612-img0005.jpg "Immunohistochemistry: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse lung using Caspase 11 antibody (clone 17D9) at 1:100 diluton. The antibody generated cytoplasmic signal with more intense staining in the bronchiolar epithelium while the stainin")
![Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - analysis of Caspase 11 in LPS stimulated bone marrow derived macrophages using anti-Caspase 11 antibody. Image from verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Western-Blot-NBP2-80612-img0001.jpg "Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - analysis of Caspase 11 in LPS stimulated bone marrow derived macrophages using anti-Caspase 11 antibody. Image from verified customer review. Image from the standard format of this antibody.")
![Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Analysis of Caspase-11 in THP-1 cell lysates that were untreated (left lane) or treated (right lane) with 100 ng/ml LPS. Image from verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Western-Blot-NBP2-80612-img0002.jpg "Western Blot: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Analysis of Caspase-11 in THP-1 cell lysates that were untreated (left lane) or treated (right lane) with 100 ng/ml LPS. Image from verified customer review. Image from the standard format of this antibody.")
![Knockdown Validated: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Casp11fl/fl and Casp11EC-/- mice (n = 3) were challenged with LPS (40 mg/kg i.p.) for 6 hours. Endothelial Cells were isolated from lungs, and western blot analysis was performed for pro-IL-1Beta, mature IL-1Beta, and caspase-1 cleavage. This image is from PMID: 28990935. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Knockdown-Validated-NBP2-80612-img0006.jpg "Knockdown Validated: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Casp11fl/fl and Casp11EC-/- mice (n = 3) were challenged with LPS (40 mg/kg i.p.) for 6 hours. Endothelial Cells were isolated from lungs, and western blot analysis was performed for pro-IL-1Beta, mature IL-1Beta, and caspase-1 cleavage. This image is from PMID: 28990935. Image from the standard format of this antibody.")
![Knockout Validated: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Caspase-11 is expressed in Casp1Null bone marrow-derived macrophages. Immunoblot analysis of caspase-1, caspase-11 and GAPDH (loading control) in unprimed WT at various times (above lane) after stimulation with IFN-beta (250U/ml), IFN-gamma (100 ng/ml) or LPS (100 ng/ml). Data are representative of two independent experiments. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45126), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Knockout-Validated-NBP2-80612-img0007.jpg "Knockout Validated: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - Caspase-11 is expressed in Casp1Null bone marrow-derived macrophages. Immunoblot analysis of caspase-1, caspase-11 and GAPDH (loading control) in unprimed WT at various times (above lane) after stimulation with IFN-beta (250U/ml), IFN-gamma (100 ng/ml) or LPS (100 ng/ml). Data are representative of two independent experiments. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45126), licensed under a CC-BY license. Image from the standard format of this antibody.")
![Knockout Validated: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - CNF1-triggered immunity requires inflammatory caspases.(A and B) IL-1beta production and maturation/secretion after treatment with CNF1, LPS or CNF1 (1 ug/ml) + LPS (100 ng/ml) for 10 h. Actin and BSA were used as loading controls. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1004732), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Knockout-Validated-NBP2-80612-img0009.jpg "Knockout Validated: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - CNF1-triggered immunity requires inflammatory caspases.(A and B) IL-1beta production and maturation/secretion after treatment with CNF1, LPS or CNF1 (1 ug/ml) + LPS (100 ng/ml) for 10 h. Actin and BSA were used as loading controls. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1004732), licensed under a CC-BY license. Image from the standard format of this antibody.")
![Knockout Validated: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - CNF1-triggered IL-1beta maturation requires activated Rac, ASC and caspase-1. Western blot analysis of the production and maturation/secretion of IL-1beta by primary macrophages following treatment with CNF1, LPS or CNF1+LPS for 10 h. Actin and BSA were used as loading controls. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1004732), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Caspase-11-Antibody-17D9-Azide-and-BSA-Free-Knockout-Validated-NBP2-80612-img0008.jpg "Knockout Validated: Caspase-11 Antibody (17D9) - Azide and BSA Free [NBP2-80612] - CNF1-triggered IL-1beta maturation requires activated Rac, ASC and caspase-1. Western blot analysis of the production and maturation/secretion of IL-1beta by primary macrophages following treatment with CNF1, LPS or CNF1+LPS for 10 h. Actin and BSA were used as loading controls. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1004732), licensed under a CC-BY license. Image from the standard format of this antibody.")
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![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
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![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![IL-18/IL-1F4 [HRP]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-18/IL-1F4 [HRP]")
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![Western Blot JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Western_Blot_6327.jpg)
![Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg)
