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Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

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ELISA: Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric) [NBP2-75045] - Samples were spiked with high concentrations of Human Calcium-sensing R/CaSR and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric) [NBP2-75045] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL

Order Details

Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CaSR in samples. No significant cross-reactivity or interference between Human CaSR and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.81%
Intra-Assay
CV% < 5.49%
Spike Recovery
89-104%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CASR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Calcium-sensing R/CaSR ELISA Kit (Colorimetric)

  • calcium-sensing receptor
  • CAR
  • CaSR
  • EIG8
  • extracellular calcium-sensing receptor
  • FHH
  • FIH
  • GPRC2A
  • GPRC2AHHC
  • HHC1
  • hypocalciuric hypercalcemia 1
  • NSHPT
  • parathyroid Ca(2+)-sensing receptor 1
  • Parathyroid cell calcium-sensing receptor
  • PCaR1
  • PCAR1MGC138441

Background

Calcium-Sensing Receptor (CaSR) is a member of the G protein-coupled receptor family. CaSR is an integral membrane protein that senses changes in the extracellular concentration of calcium ions and controls the release of parathyroid hormone. The activity of the calcium-sensing receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.

Mutations that inactivate CaSR lead to familial hypocalciuric hypercalcemia, whereas mutations that activate CaSR cause autosomal dominant hypocalcemia. Calcium-Sensing Receptor antibodies are useful tools for parathyroid studies and research on cellular calcium homeostasis regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CASR