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BTK [p Tyr223] Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-78295AF350
Alexa Fluor 405
NBP1-78295AF405
Alexa Fluor 488
NBP1-78295AF488
Alexa Fluor 532
NBP1-78295AF532
Alexa Fluor 594
NBP1-78295AF594
Alexa Fluor 647
NBP1-78295AF647
Alexa Fluor 700
NBP1-78295AF700
Alexa Fluor 750
NBP1-78295AF750
Allophycocyanin
NBP1-78295APC
CoraFluor 1
NBP1-78295CL1
DyLight 350
NBP1-78295UV
DyLight 405
NBP1-78295V
DyLight 488
NBP1-78295G
DyLight 550
NBP1-78295R
DyLight 594
NBP1-78295DL594
DyLight 650
NBP1-78295C
DyLight 680
NBP1-78295FR
DyLight 755
NBP1-78295IR
FITC
NBP1-78295F
HRP
NBP1-78295H
Janelia Fluor 525
NBP1-78295JF525
Janelia Fluor 549
NBP1-78295JF549
Janelia Fluor 646
NBP1-78295JF646
Janelia Fluor 669
NBP1-78295JF669
PE
NBP1-78295PE
PerCP
NBP1-78295PCP
Unconjugated
NBP1-78295
mFluor Violet 450 SE
NBP1-78295MFV450
mFluor Violet 500 SE
NBP1-78295MFV500
mFluor Violet 610 SE
NBP1-78295MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-78295
BSA Free
NBP1-78295SS
BSA Free
NBP1-78295
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BTK [p Tyr223] Antibody [Biotin] Summary

Immunogen
A synthetic phosphorylated peptide made to a peptide surrounding amino acid 223 of the human BTK protein [UniProt Q06187]
Modification
p Tyr223
Localization
Cytoplasm. Membrane; Peripheral membrane protein. Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BTK
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BTK [p Tyr223] Antibody [Biotin]

  • Agammaglobulinaemia tyrosine kinase
  • AGMX1
  • AGMX1MGC126262
  • AT
  • ATKIMD1
  • B-cell progenitor kinase
  • BPK
  • Bruton agammaglobulinemia tyrosine kinase
  • Bruton tyrosine kinase
  • BTK
  • dominant-negative kinase-deficient Brutons tyrosine kinase
  • EC 2.7.10
  • EC 2.7.10.2
  • IMD1
  • PSCTK1
  • tyrosine-protein kinase BTK
  • XLA
  • XLAMGC126261

Background

Bruton's tyrosine kinase (BTK) takes part in B-cell ontogeny, differentiation, and signaling. A kinase member of the Tec family, it's signaling leads to cytoskeletal changes, as well as calcium, NF kappa B, and NFAT signaling. Defects in the gene cause X-linked agammaglobulinemia (XLA). Autophosphorylation can occur on Tyr-223 and Tyr-551. pY223 activates the protein that is inhibited by IBTK, and creates a docking site for SH2 domain proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BTK