BTF3 Antibody Summary
Immunogen |
E. coli-derived recombinant human BTF3 Lys46-Asn206 Accession # P20290 |
Specificity |
Detects human, mouse and rat BTF3 in Western blots. |
Source |
N/A |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Goat |
Gene |
BTF3 |
Purity Statement |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 5-15 ug/mL
- Western Blot 0.5 ug/mL
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Publications |
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Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Reconstitution Instructions |
Reconstitute at 0.2 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BTF3 Antibody
Background
BTF3 (basic transcription factor 3; also nascent polypeptide-associated complex beta /NACB) is a member of the NAC-beta family of transcription factors. It is ubiquitously expressed and involved in the initiation of gene transcription by RNA polymerase. BTF3 also appears to impact estrogen receptor alpha (ER alpha ) stimulation of gene activation by binding to both ligand activated and non-ligand activated ER alpha . Human BTF3 is 206 amino acids (aa) in length and contains one potential phosphorylation site at Ser30, and a NACB domain (aa 82-147). There are multiple potential splice variants. Two variants show alternate start sites, one at Met45 (called BTF3b), and another at Met108.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for BTF3 Antibody (AF5747)(2)
Showing Publications 1 -
2 of 2.
Publications using AF5747 |
Applications |
Species |
Long Liu, Yanfeng Fan, Debiao Zhao, Jason Ioannidis, Daoqing Gong, Michael Clinton Expression Profile of Chicken Sex Chromosome Gene<b><i> BTF3 </i></b>is Linked to Gonadal Phenotype Sexual Development 1/31/2020 [PMID: 32155647] |
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Aydan Bulut-Karslioglu, Trisha A. Macrae, Juan A. Oses-Prieto, Sergio Covarrubias, Michelle Percharde, Gregory Ku et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output Cell Stem Cell 2018-03-01 [PMID: 29499153] |
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Secondary Antibodies
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