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Recombinant Human BRCA2 GST (N-Term) Protein

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SDS-Page: BRCA2 Recombinant Protein [H00000675-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, MA, AP

Order Details

Recombinant Human BRCA2 GST (N-Term) Protein Summary

Description
A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 3319-3418 of Human BRCA2

Source: Wheat Germ (in vitro)

Amino Acid Sequence: SPQMTPFKKFNEISLLESNSIADEELALINTQALLSGSTGEKQFISVSESTRTAPTSSEDYLRLKRRCTTSLIKEQESSQASTEECEKNKQDTITTKKYI

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Partial Recombinant Protein
Gene
BRCA2
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunoaffinity Purification
  • Immunohistochemistry
  • Protein Array
  • Western Blot
Theoretical MW
36.63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
H00000675-Q01 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human BRCA2 GST (N-Term) Protein

  • BRCA1/BRCA2-containing complex, subunit 2
  • BRCA2
  • BRCC2
  • breast and ovarian cancer susceptibility gene, early onset
  • breast cancer 2 tumor suppressor
  • breast cancer 2, early onset
  • breast cancer susceptibility protein BRCA2
  • breast cancer type 2 susceptibility protein
  • BROVCA2
  • FACD
  • FAD
  • FAD1
  • FANCB
  • FANCD
  • FANCD1Fanconi anemia, complementation group D1
  • Fanconi anemia group D1 protein
  • GLM3
  • PNCA2

Background

BRCA 2 is localized to chromosome 13q12 - q13. Loss of heterozygosity of 13q is observed in 25% of sporadic breast tumors, which indicates that BRCA 2 might be a tumor suppressor gene. BRCA 2 confers only a low ovarian cancer risk. Reportedly, in MCF-10F (normal breast epithelial cell line) and MCF-7 (breast cancer cell line) cells the expression of BRCA 2 RNA lowers in G0 and early G1 phases then gets up-regulated at the G1 / S phase junction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for BRCA2 Recombinant Protein (H00000675-Q01)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IF/IHC.


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IF/IHC
(1)
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Human
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BRCA2 Recombinant Protein (H00000675-Q01) (0)

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Bioinformatics

Gene Symbol BRCA2