We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.
The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M) are of particular interest to cancer groups, as defects in these proteins are known to cause Fanconi's anemia, which carries a higher risk of cancer developing. The complex is also associated with the breast cancer susceptibility gene BRCA2. Recently, we extended our antibody catalog with the addition of several SDIX GAT (Genome Antibody Technology) reagents.
Under normal circumstances, the core complex is activated in response to DNA damage, the trigger being the cessation of replication. The proteins interact with BRCA2 by addition of ubiquitin (ubiquitinization) which then facilitates repair. Recent studies have been able to look at this process in depth.
Immunocytochemistry: Fanconi Anemia Antibody Pack
It is thought the 8 proteins in the core complex migrate from the cytoplasm to the nucleus following nuclear localization signalling of FANCA and FANCE. Assembly of the complex is activated by replication stress, in particular DNA damage caused by cross-linking agents or oxidative stress caused by ROS (reactive oxygen species). The assembled complex then triggers FANCL, an E3 ubiquitin ligase protein which monoubiquitinates FANCD2. This then interacts with the BRCA1/BRCA2 complex.
It is now known that there are several similar complexes involved in DNA damage recognition and repair activation, which interact with the FA genes. Recently, researchers identified two novel proteins – christened MHF1 and MHF2 – associated with the core protein FANCM. Antibody supplierslike us at Novus Biologicals are constantly revising our catalogs as new discoveries are made.
Novus Biologicals offers many Fanconi anemia reagents for your research needs including:
