![Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) - Azide and BSA Free [NBP2-72407] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Bestrophin 3.](http://images.novusbio.com/fullsize/Bestrophin-3-Antibody-OTI2H3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72407-img0004.jpg "Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) - Azide and BSA Free [NBP2-72407] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Bestrophin 3.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, CyTOF-ready |
| Clone | OTI2H3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human BEST3 (NP_689652) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BEST3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 50.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
![Immunohistochemistry Neurophysin II/Arg-vasopressin Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af009_human-neurophysin-ii-affinity-purified-polyclonal-ab-immunohistochemistry-2812202013195.jpg)
Secondary Antibodies |
Isotype Controls |
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"I can see clearly now": Targeting Bestrophin 1 to treat Bestrophinopathies Encoded by the VMD2 gene on chromosome 11q13 Bestrophin 1 is the prototypic member of the RFP family of proteins which are more commonly called "bestrophins". The protein family was originally identified in Caenorhabditis elegans based on a conserved ... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BEST3 |
![Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) - Azide and BSA Free [NBP2-72407] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Bestrophin 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Bestrophi](http://images.novusbio.com/fullsize/Bestrophin-3-Antibody-OTI2H3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72407-img0001.jpg "Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) - Azide and BSA Free [NBP2-72407] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Bestrophin 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Bestrophi")
![Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) - Azide and BSA Free [NBP2-72407] - Analysis of Hela cells, using anti-Bestrophin 3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Bestrophin-3-Antibody-OTI2H3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72407-img0002.jpg "Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) - Azide and BSA Free [NBP2-72407] - Analysis of Hela cells, using anti-Bestrophin 3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) - Azide and BSA Free [NBP2-72407] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Bestrophin 3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Bestrophin-3-Antibody-OTI2H3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72407-img0003.jpg "Flow Cytometry: Bestrophin 3 Antibody (OTI2H3) - Azide and BSA Free [NBP2-72407] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Bestrophin 3 antibody, and then analyzed by flow cytometry.")
![N/A Trappin-2/Elafin/Skalp [Biotin]](https://images.novusbio.com/images2/DATA_Trappin2_DY1747_ELISA_1708.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg)
![Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")