![Western Blot: Bestrophin 3 Antibody (2H3) [NBP2-03253] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Bestrophin 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Bestrophin 3.](http://images.novusbio.com/fullsize/Bestrophin-3-Antibody-2H3-Western-Blot-NBP2-03253-img0008.jpg "Western Blot: Bestrophin 3 Antibody (2H3) [NBP2-03253] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Bestrophin 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Bestrophin 3.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | OTI2H3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.98 mg/ml |
| Immunogen | Full length human recombinant protein of human BEST3 (NP_689652) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BEST3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 50.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.98 mg/ml |
| Purity | Immunogen affinity purified |
![Immunohistochemistry Neurophysin II/Arg-vasopressin Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af009_human-neurophysin-ii-affinity-purified-polyclonal-ab-immunohistochemistry-2812202013195.jpg)
Secondary Antibodies |
Isotype Controls |
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"I can see clearly now": Targeting Bestrophin 1 to treat Bestrophinopathies Encoded by the VMD2 gene on chromosome 11q13 Bestrophin 1 is the prototypic member of the RFP family of proteins which are more commonly called "bestrophins". The protein family was originally identified in Caenorhabditis elegans based on a conserved ... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Bestrophin 3.](http://images.novusbio.com/fullsize/Bestrophin-3-Antibody-2H3-Immunocytochemistry-Immunofluorescence-NBP2-03253-img0007.jpg "Immunocytochemistry/Immunofluorescence: Bestrophin 3 Antibody (OTI2H3) [NBP2-03253] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Bestrophin 3.")
![Flow Cytometry: Bestrophin 3 Antibody (2H3) [NBP2-03253] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Bestrophin 3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Bestrophin-3-Antibody-2H3-Flow-Cytometry-NBP2-03253-img0006.jpg "Flow Cytometry: Bestrophin 3 Antibody (2H3) [NBP2-03253] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Bestrophin 3 antibody, and then analyzed by flow cytometry.")
![Flow Cytometry: Bestrophin 3 Antibody (2H3) [NBP2-03253] - Analysis of Hela cells, using anti-Bestrophin 3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Bestrophin-3-Antibody-2H3-Flow-Cytometry-NBP2-03253-img0005.jpg "Flow Cytometry: Bestrophin 3 Antibody (2H3) [NBP2-03253] - Analysis of Hela cells, using anti-Bestrophin 3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_6973.jpg)
![Immunohistochemistry CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CFTR_MAB25031_Immunohistochemistry_9671.jpg)
![Immunoprecipitation CFTR Antibody (24-1) [Unconjugated] - C-terminus](https://images.novusbio.com/images/mab25031_human-cftr-c-terminus-mab-clone-24-1-41202412513815.jpg)
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")