![Western Blot: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Atrial Natriuretic Peptide/ANP antibody [GT1068] (NBP3-13605) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-GT1068-Western-Blot-NBP3-13605-img0001.jpg "Western Blot: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Atrial Natriuretic Peptide/ANP antibody [GT1068] (NBP3-13605) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | GT1068 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Full length human Atrial Natriuretic Peptide/ANP recombinant protein. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NPPA |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC, ICC/IF-Assay dependent |
| Theoretical MW | 17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS pH7, 20% Glycerol |
| Preservative | No Preservative |
| Purity | Protein G purified |
![Immunohistochemistry Neurophysin II/Arg-vasopressin Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af009_human-neurophysin-ii-affinity-purified-polyclonal-ab-immunohistochemistry-2812202013195.jpg)
![Immunohistochemistry ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Immunohistochemistry_6703.jpg)
![Western Blot ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Flow_Cytometry_20028.jpg)
![Simple Western ACE/CD143 Antibody [Unconjugated]](https://images.novusbio.com/images2/ACE_AF1513_Simple_Western_20109.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NPPA |
![Immunocytochemistry/Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ANP stained by ANP antibody [1068] iluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-GT1068-Immunocytochemistry-Immunofluorescence-NBP3-13605-img0002.jpg "Immunocytochemistry/Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ANP stained by ANP antibody [1068] iluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.")
![Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - Mouse heart. ANP stained by ANP antibody [1068] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-GT1068-Immunohistochemistry-Paraffin-NBP3-13605-img0003.jpg "Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - Mouse heart. ANP stained by ANP antibody [1068] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
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![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-44202415433122.jpg)
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![N/A Endothelin-1 [HRP]](https://images.novusbio.com/images2/Endothelin-1_QET00_ELISA_432.jpg)
![N/A Endothelin-1 [HRP]](https://images.novusbio.com/images2/DATA_Endothelin1_QET00_ELISA_1074.jpg)
![N/A Angiogenin [HRP]](https://images.novusbio.com/images2/Angiogenin_DAN00_ELISA_28.jpg)
![N/A Angiogenin [HRP]](https://images.novusbio.com/images2/DATA_Angiogenin_DAN00_ELISA_571.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")