![Western Blot: ATG4D Antibody [NBP2-41308] - Analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Western-Blot-NBP2-41308-img0001.jpg "Western Blot: ATG4D Antibody [NBP2-41308] - Analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 ug/ml.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 17 amino acid peptide near the amino terminus of human ATG4D. The immunogen is located within the first 50 amino acids of ATG4D. Amino Acid Squence: SPEDARRRPEARRPRG |
| Specificity | At least two isoforms of ATG4D are known to exist; this antibody will detect only the larger isoform. ATG4D is predicted to not cross-react with other ATG4 proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ATG4D |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
| Publication using NBP2-41308 | Applications | Species |
|---|---|---|
| Wang X, Zeng J, Wang X et al. 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside induces autophagy of liver by activating PI3K/Akt and Erk pathway in prediabetic rats BMC Complement Med Ther 2020-06-08 [PMID: 32513151] (WB, Rat) | WB | Rat |
Secondary Antibodies |
Isotype Controls |
Research Areas for ATG4D Antibody (NBP2-41308)Find related products by research area.
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ATG4D - A regulator of autophagy and apoptosis Autophagy is an essential cellular process whereby damaged proteins and organelles are degraded and recycled. Autophagy, while happening constantly at a basal level, is tightly regulated and can be further induced under cellular stress. One of the ... Read full blog post. |
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| Gene Symbol | ATG4D |
![Immunohistochemistry: ATG4D Antibody [NBP2-41308] - Human testis tissue with ATG4D antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunofluorescence-NBP2-41308-img0003.jpg "Immunohistochemistry: ATG4D Antibody [NBP2-41308] - Human testis tissue with ATG4D antibody at 20 ug/ml.")
![Immunohistochemistry: ATG4D Antibody [NBP2-41308] - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-NBP2-41308-img0002.jpg "Immunohistochemistry: ATG4D Antibody [NBP2-41308] - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 ug/ml.")
![Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308] - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp2-41308_rabbit-polyclonal-atg4d-antibody-2711202419592031.jpg "Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308] - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 u/ml.")
![Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308] - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 u/ml.](http://images.novusbio.com/fullsize/nbp2-41308_rabbit-polyclonal-atg4d-antibody-2711202419241347.jpg "Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308] - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 u/ml.")
![Immunocytochemistry/ Immunofluorescence: ATG4D Antibody - BSA Free [NBP2-41308] - Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-41308_rabbit-polyclonal-atg4d-antibody-2711202420115547.jpg "Immunocytochemistry/ Immunofluorescence: ATG4D Antibody - BSA Free [NBP2-41308] - Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 u/ml.")
![Immunocytochemistry/ Immunofluorescence: ATG4D Antibody - BSA Free [NBP2-41308] - Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-41308_rabbit-polyclonal-atg4d-antibody-2711202420175112.jpg "Immunocytochemistry/ Immunofluorescence: ATG4D Antibody - BSA Free [NBP2-41308] - Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 u/ml.")
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")