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ATF4 Antibody (S360A-24) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications ICC/IF, IHC
Clone
S360A-24
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

ATF4 Antibody (S360A-24) [Janelia Fluor® 646] Summary

Immunogen
Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.
Localization
Cytoplasm , Cell Membrane , Nucleus
Specificity
Less then 50% identity with ATF5.Detects approx 60kDa. Does not cross-react with ATF5.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATF4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for ATF4 Antibody (S360A-24) [Janelia Fluor® 646]

  • activating transcription factor 4 (tax-responsive enhancer element B67)
  • Activating transcription factor 4
  • ATF4
  • cAMP-dependent transcription factor ATF-4
  • cAMP-responsive element-binding protein 2
  • CREB-2DNA-binding protein TAXREB67
  • cyclic AMP-dependent transcription factor ATF-4
  • Cyclic AMP-responsive element-binding protein 2
  • TAXREB67
  • TAXREB67CREB2cAMP response element-binding protein 2
  • Tax-responsive enhancer element-binding protein 67
  • TXREB

Background

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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CENPF Antibodies as Potential Cancer Markers
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Bioinformatics

Gene Symbol ATF4