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ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
Ser29-Lys359 (Val151Ile)
Accession # Q02083
Specificity
Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant mouse ASAHL is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NAAA
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF4494 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody

  • Acid ceramidase-like protein
  • ASAHL
  • ASAH-like protein
  • EC 3.5.1.-
  • NAAA
  • N-acylethanolamine acid amidase
  • Nacylethanolaminehydrolyzing Acid Amidase
  • N-acylethanolamine-hydrolyzing acid amidase
  • N-acylsphingosine amidohydrolase (acid ceramidase)-like
  • N-acylsphingosine amidohydrolase-like
  • PLT

Background

ASAHL (N-acylsphingosine-amidohydrolase-like; N acylethanolamine hydrolyzine acid amidase; also acid ceramidase like protein) is a 30 kDa member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359 amino acid (aa) preproprecursor that contains a 28 aa signal sequence, a 96 aa prosegment, and a 30 31 kDa, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa 324 359, while another has a two aa substitution for aa 198 359. The human proform (aa 29 359) is 78% aa identical to mouse ASAHL proform.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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    Publications for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (AF4494)(1)

    We have publications tested in 1 confirmed species: Human.

    We have publications tested in 2 applications: IHC, Western Blot.


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    IHC
    (1)
    Western Blot
    (1)
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    Human
    (1)
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    Bioinformatics

    Gene Symbol NAAA
    Uniprot