Home » ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase » ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibodies » ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3)
Novus Biologicals products are now on bio-techne.com

ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - NAAA monoclonal antibody (M01), clone 5E3 Analysis of ASAHL expression in NIH/3T3.
Western Blot: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - NAAA monoclonal antibody (M01), clone 5E3 Analysis of ASAHL expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - Analysis of monoclonal antibody to NAAA on NIH/3T3 cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) [H00027163-M01] - Detection limit for recombinant GST tagged NAAA is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
5E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) Summary

Immunogen
NAAA (NP_055250, 36 a.a. ~ 104 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLIGKVVLELERFLPQPFTGEIRGMCD
Localization
Lysosome
Specificity
ASAHL - N-acylsphingosine amidohydrolase (acid ceramidase)-like
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NAAA
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3)

  • Acid ceramidase-like protein
  • ASAHL
  • ASAH-like protein
  • EC 3.5.1.-
  • NAAA
  • N-acylethanolamine acid amidase
  • Nacylethanolaminehydrolyzing Acid Amidase
  • N-acylethanolamine-hydrolyzing acid amidase
  • N-acylsphingosine amidohydrolase (acid ceramidase)-like
  • N-acylsphingosine amidohydrolase-like
  • PLT

Background

ASAHL (N-acylsphingosine amidohydrolase (acid ceramidase)-like) encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. It degrades bioactive fatty acid amides to their corresponding acids and also exhibits weak hydrolytic activity against the ceramides N-lauroylsphingosine and N-palmitoylsphingosine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

P-Selectin/CD62P
137-PS
P-Selectin/CD62P
(46 Publications)
Species: Hu
Applications: BA
FAAH Antibody (4H8)
H00002166-M07
Western Blot FAAH Antibody (4H8)
ELISA FAAH Antibody (4H8)
Western Blot FAAH Antibody (4H8)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, WB
CCL21/6Ckine
[Unconjugated]
366-6C
CCL21/6Ckine [Unconjugated]
(35 Publications)
Species: Hu
Applications: BA
CCL19/MIP-3 beta
[Unconjugated]
361-MI
CCL19/MIP-3 beta [Unconjugated]
(41 Publications)
Species: Hu
Applications: BA
HLA DPB1 Antibody
NBP1-89688
Immunohistochemistry-Paraffin HLA DPB1 Antibody
Immunohistochemistry-Paraffin HLA DPB1 Antibody
Immunohistochemistry-Paraffin HLA DPB1 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
Prothrombin
Antibody
NBP1-58268
Immunohistochemistry-Paraffin Prothrombin Antibody
Immunohistochemistry-Paraffin Prothrombin Antibody
Western Blot Prothrombin Antibody
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
IL-2 [Unconjugated]
202-IL
Bioactivity IL-2 [Unconjugated]
(4 Reviews)
(377 Publications)
Species: Hu
Applications: BA
FA2H Antibody
(OTI2E3)
NBP2-45564
Western Blot FA2H Antibody (OTI2E3)
Immunofluorescence FA2H Antibody (OTI2E3)
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Monoglyceride
Lipase Antibody (OTI2
NBP2-00735
Western Blot Monoglyceride Lipase Antibody (OTI2B11)
Immunocytochemistry/ Immunofluorescence Monoglyceride Lipase Antibody (OTI2B11)
Flow Cytometry Monoglyceride Lipase Antibody (OTI2B11)
(1 Publication)
Species: Hu, Pm
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Sialin/SLC17A5
Antibody
NBP2-20383
Immunohistochemistry-Paraffin Sialin/SLC17A5 Antibody
Western Blot Sialin/SLC17A5 Antibody
Immunocytochemistry/ Immunofluorescence Sialin/SLC17A5 Antibody
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
CCR7 Antibody
(150503) [Unconjugate
MAB197
Immunocytochemistry CCR7 Antibody (150503) [Unconjugated]
Immunohistochemistry CCR7 Antibody (150503) [Unconjugated]
Neutralization CCR7 Antibody (150503) [Unconjugated]
(1 Review)
(103 Publications)
Species: Hu
Applications: CyTOF-reported, Dual ISH-IHC, Flow, ICC, IHC, Neut
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(54 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Integrin alpha
2b/CD41 Antibody (745 ...
MAB7616
Western Blot Integrin alpha 2b/CD41 Antibody (745201) [Unconjugated]
Flow Cytometry Integrin alpha 2b/CD41 Antibody (745201) [Unconjugated]
Flow Cytometry Integrin alpha 2b/CD41 Antibody (745201) [Unconjugated]
(6 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, WB
Tenascin C
Antibody (4C8MS) - BS
NB110-68136
Flow Cytometry Tenascin C Antibody (4C8MS) - BSA Free
Immunocytochemistry/ Immunofluorescence Tenascin C Antibody (4C8MS) - BSA Free
Flow Cytometry Tenascin C Antibody (4C8MS) - BSA Free
(2 Reviews)
(26 Publications)
Species: Fe, Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
RNF130 Antibody
NBP1-81858
Immunohistochemistry-Paraffin RNF130 Antibody
Western Blot RNF130 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
Desmoplakin
Antibody
NBP2-48836
Immunohistochemistry-Paraffin Desmoplakin Antibody
Immunohistochemistry-Paraffin Desmoplakin Antibody
Immunohistochemistry-Paraffin Desmoplakin Antibody
(2 Publications)
Species: Ca, Hu
Applications: ICC/IF, IHC, IHC-P

Publications for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (H00027163-M01) (0)

There are no publications for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (H00027163-M01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (H00027163-M01) (0)

There are no reviews for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (H00027163-M01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (H00027163-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Products

Blogs on ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase

There are no specific blogs for ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
As an alternative, please consider our next generation Recombinant Human IL-2 (BT-002). It has equivalent bioactivity to Recombinant Human IL-2 (Catalog # 202-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-2 proteins are measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.
IL-2 [Unconjugated]
202-IL-010

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody (5E3) and receive a gift card or discount.


Bioinformatics

Gene Symbol NAAA
Entrez
OMIM
Uniprot