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ASAH1 Antibody [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 669

Order Details

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ASAH1 Antibody [Janelia Fluor® 669] Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human ASAH1. The immunogen is located within amino acids 240 - 290 of ASAH1. Amino Acid Squence: LENSTSYEEAKNLLTK
Specificity
Multiple isoforms of ASAH1 are known to exist.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASAH1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine: (88%)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for ASAH1 Antibody [Janelia Fluor® 669]

  • ACDase
  • ACEC 3.5.1.23
  • Acid CDase
  • acid ceramidase
  • Acylsphingosine deacylase
  • ASAH
  • FLJ21558
  • FLJ22079
  • N-acylsphingosine amidohydrolase (acid ceramidase) 1
  • N-acylsphingosine amidohydrolase
  • PHP
  • PHP32N-acylsphingosine amidohydrolase (acid ceramidase)
  • Putative 32 kDa heart protein

Background

ASAH1 encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ASAH1