![Western Blot: Arylsulfatase B/ARSB Antibody [NBP1-06021] - (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Arylsulfatase-B-ARSB-Antibody-Western-Blot-NBP1-06021-img0004.jpg "Western Blot: Arylsulfatase B/ARSB Antibody [NBP1-06021] - (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-KLARGHTNGTKPLD corresponding to internal region according to NP_000037.2, NP_942002.1. |
| Epitope | C-KLARGHTNGTKPLD |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_000037.2; NP_942002.1). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ARSB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 45 kDa band observed in human heart lysates (calculated MW of 46.0 kDa band according to NP_942002.1). An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human skeletal muscle shows striated pattern staining of majority of muscular fibres. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-06021 | Applications | Species |
|---|---|---|
| Bhattacharyya S, Look D, Tobacman JK. Increased arylsulfatase B activity in cystic fibrosis cells following correction of CFTR. Clin Chim Acta 2007-05-01 [PMID: 17324393] |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | ARSB |
| Entrez |
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| Uniprot |
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![Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-06021] - Arylsulfatase B/ARSB Antibody [NBP1-06021] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Arylsulfatase-B-ARSB-Antibody-Immunohistochemistry-Paraffin-NBP1-06021-img0007.jpg "Immunohistochemistry-Paraffin: Arylsulfatase B/ARSB Antibody [NBP1-06021] - Arylsulfatase B/ARSB Antibody [NBP1-06021] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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