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ARF5 Antibody (1B6)

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Western Blot: ARF5 Antibody (1B6) [H00000381-M02] - Analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M02), clone 1B6.Lane 1: ARF5 transfected lysate(20.5 KDa).Lane 2: ...read more
ELISA: ARF5 Antibody (1B6) [H00000381-M02] - Detection limit for recombinant GST tagged ARF5 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ARF5 Antibody (1B6) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ARF5 (AAH03043, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR
Specificity
ARF5 - ADP-ribosylation factor 5 (1B6)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ARF5
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ARF5 Antibody (1B6)

  • ADP-ribosylation factor 5

Background

ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00000381-M02
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Applications: WB, ELISA

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ARF5 Antibody (H00000381-M02). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for antibodies against human ARF4 and ARF5 and I need to be able to distinguish between the two proteins. Do you have antibodies specific for each protein that won't cross react with the other protein?
    • I checked the sequence similarity between ARF4 and ARF5, and there is very high homology between the two proteins. We have not specifically tested our antibodies for cross-reactivity between the two, but I think it's likely that they will.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARF5
Entrez
Uniprot