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Aquaporin 1/AQP1 Antibody (OTI2D10)

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Western Blot: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Analysis of extracts (10ug) from 3 different cell lines by using anti-AQP1 monoclonal antibody at 1:200 dilution.
Immunocytochemistry/ Immunofluorescence: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Aquaporin-1.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Staining of paraffin-embedded Human lymphoma tissue using anti-Aquaporin-1 mouse monoclonal antibody.
Flow Cytometry: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Aquaporin-1 Antibody (2D10) [NBP2-03568] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) ...read more
Western Blot: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Aquaporin-1 Antibody (2D10) [NBP2-03568] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Aquaporin-1 (Right ...read more
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Aquaporin-1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Staining of paraffin-embedded Human Kidney tissue using anti-Aquaporin-1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (OTI2D10) [NBP2-03568] - Staining of paraffin-embedded Human lung tissue using anti-Aquaporin-1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.47 mg/ml

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Aquaporin 1/AQP1 Antibody (OTI2D10) Summary

Immunogen
Full length human recombinant protein of human AQP1 (NP_932766) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AQP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
28.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Brain Cerebral Meninges Membrane Tissue Lysate (Adult Membrane Normal)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.47 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aquaporin 1/AQP1 Antibody (OTI2D10)

  • 28-kDa
  • AQP1
  • AQP-1
  • AQP-CHIP
  • aquaporin 1 (channel-forming integral protein, 28kDa)
  • aquaporin 1 (Colton blood group)
  • Aquaporin 1
  • Aquaporin-CHIP
  • CHIP28
  • CHIP2828kDa, CO blood group)
  • CO
  • MGC26324

Background

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Aquaporin 1/AQP1 Antibody (NBP2-03568). (Showing 1 - 1 of 1 FAQ).

  1. Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.
    • A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AQP1
Entrez