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Rat Apolipoprotein B/ApoB ELISA Kit (Colorimetric)

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ELISA: Rat Apolipoprotein B/ApoB ELISA Kit (Colorimetric) [NBP2-82121] - Samples were spiked with high concentrations of Rat Apolipoprotein B/ApoB and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Rat Apolipoprotein B/ApoB ELISA Kit (Colorimetric) [NBP2-82121] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
31.25 - 2000 ng/mL
Sensitivity
18.75 ng/mL

Order Details

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Rat Apolipoprotein B/ApoB ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Apolipoprotein B/ApoBin samples. No Significant cross-reactivity or interference between Rat Apolipoprotein B/ApoB and analogues was observed.
Standard Curve Range
31.25 - 2000 ng/mL
Sensitivity
18.75 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 6%
Spike Recovery
average = 100% for serum; average = 95% for EDTA plasma; average = 93% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
APOB

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat Apolipoprotein B/ApoB ELISA Kit (Colorimetric)

  • Apo B-100
  • APOB
  • ApoB-100
  • ApoB-48
  • apolipoprotein B (including Ag(x) antigen)
  • Apolipoprotein B
  • apolipoprotein B-100
  • apolipoprotein B48
  • FLDB
  • LDLCQ4
  • mutant Apo B 100

Background

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol APOB