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Human Apolipoprotein A5 ELISA Kit (Colorimetric)

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ELISA: Human Apolipoprotein A5 ELISA Kit (Colorimetric) [NBP3-18758] - Samples were spiked with high concentrations of Human Apolipoprotein A5 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Apolipoprotein A5 ELISA Kit (Colorimetric) [NBP3-18758] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.5-4000 pg/mL
Sensitivity
37.5 pg/mL

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Human Apolipoprotein A5 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Apolipoprotein A5 in samples. No significant cross-reactivity or interference between Human Apolipoprotein A5 and analogues was observed.
Standard Curve Range
62.5-4000 pg/mL
Sensitivity
37.5 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.18
Intra-Assay
%CV < 6.00
Spike Recovery
89-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
APOA5

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Apolipoprotein A5 ELISA Kit (Colorimetric)

  • APOAV
  • apo-AV
  • APOA-V
  • Apolipoprotein A5
  • apolipoprotein A-V
  • FLJ97995
  • MGC126836
  • RAP3MGC126838
  • Regeneration-associated protein 3

Background

Apolipoprotein A5 (ApoA5) is a minor apolipoprotein, predominantly associated with HDL and and occasionally with VLDL and chylomicrons. ApoA-5 is a useful indicator of plasma triglyceride levels, a major risk factor for coronary artery disease.

Apo A5 is expressed in the plasma and the liver, where it is involved in the early phase of liver regeneration.

APOA5 mutations can lead to increaased susceptibility to familial hypertriglyceridemia (FHTR), a common inherited disorder where VLDL concentrations are elevated in the plasma. This disorder increases the risk of heart disease, obesity, and pancreatitis. APOA5 mutations can also cause hyperlipoproteinemia type 5 (HLPP5), characterized by increased amounts of chylomicrons/VLDL and decreased amounts of LDL/HDL in the plasma. This disorder often leads to a wide array of syndromes, including insulin-dependent diabetes mellitus, contraceptive steroids, alcohol abuse, and glycogen storage disease type 1A

ApoA5 antibodies are useful for studying lipoprotein processing and a variety of cardiac diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol APOA5