Human Apolipoprotein A5 ELISA Kit (Chemiluminescence) Summary
Specificity |
This kit recognizes Human ApoA5 in samples. No significant cross-reactivity or interference between Human ApoA5 and analogues was observed. |
Standard Curve Range |
15.63-1000 pg/mL |
Sensitivity |
9.38 pg/mL |
Assay Type |
Sandwich-CLIA |
Inter-Assay |
CV% < 9.47% |
Intra-Assay |
CV% < 9.77% |
Spike Recovery |
85-111% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Chemiluminescence) |
Gene |
APOA5 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
|
Alternate Names for Human Apolipoprotein A5 ELISA Kit (Chemiluminescence)
Background
Apolipoprotein A5 (ApoA5) is a minor apolipoprotein, predominantly associated with HDL and and occasionally with VLDL and chylomicrons. ApoA-5 is a useful indicator of plasma triglyceride levels, a major risk factor for coronary artery disease.
Apo A5 is expressed in the plasma and the liver, where it is involved in the early phase of liver regeneration.
APOA5 mutations can lead to increaased susceptibility to familial hypertriglyceridemia (FHTR), a common inherited disorder where VLDL concentrations are elevated in the plasma. This disorder increases the risk of heart disease, obesity, and pancreatitis. APOA5 mutations can also cause hyperlipoproteinemia type 5 (HLPP5), characterized by increased amounts of chylomicrons/VLDL and decreased amounts of LDL/HDL in the plasma. This disorder often leads to a wide array of syndromes, including insulin-dependent diabetes mellitus, contraceptive steroids, alcohol abuse, and glycogen storage disease type 1A
ApoA5 antibodies are useful for studying lipoprotein processing and a variety of cardiac diseases.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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