Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit Summary
Standard Curve Range |
0.63 - 40 ng/mL |
Sensitivity |
0.2 ng/mL |
Inter-Assay |
%CV < 9.8 |
Intra-Assay |
%CV < 6.0 |
Spike Recovery |
89 - 96% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit |
Gene |
APOA1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated detection mAb (HDL 44)
- Buffers for washing and dilution and standard reconstitution
- Human apoA1 ELISA standard
- Pre-coated ELISA strip plates (mAb HDL 110)
- Standard reconstitution buffer
- Streptavidin-HRP
- Substrate (TMB)
|
Alternate Names for Non-human Primate Apolipoprotein A-I/ApoA1 ELISA Kit
Background
Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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