Mouse Apolipoprotein A-I/ApoA1 ELISA Kit (Chemiluminescence) Summary
Specificity |
This kit recognizes Mouse APO-A1 in samples. No significant cross-reactivity or interference between Mouse APO-A1 and analogues was observed. |
Standard Curve Range |
1.56-100 ng/mL |
Sensitivity |
0.94 ng/mL |
Assay Type |
Sandwich-CLIA |
Inter-Assay |
CV% < 9.39% |
Intra-Assay |
CV% < 8.97% |
Spike Recovery |
90-113% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Chemiluminescence) |
Gene |
APOA1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
|
Alternate Names for Mouse Apolipoprotein A-I/ApoA1 ELISA Kit (Chemiluminescence)
Background
Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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