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Mouse Apolipoprotein A-I/ApoA1 ELISA Kit (Chemiluminescence)

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ELISA: Mouse Apolipoprotein A-I/ApoA1 ELISA Kit (Chemiluminescence) [NBP2-68241] - Samples were spiked with high concentrations of Mouse Apolipoprotein A-I/ApoA1 and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Mouse Apolipoprotein A-I/ApoA1 ELISA Kit (Chemiluminescence) [NBP2-68241] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
1.56-100 ng/mL
Sensitivity
0.94 ng/mL

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Mouse Apolipoprotein A-I/ApoA1 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Mouse APO-A1 in samples. No significant cross-reactivity or interference between Mouse APO-A1 and analogues was observed.
Standard Curve Range
1.56-100 ng/mL
Sensitivity
0.94 ng/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 9.39%
Intra-Assay
CV% < 8.97%
Spike Recovery
90-113%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
APOA1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Mouse Apolipoprotein A-I/ApoA1 ELISA Kit (Chemiluminescence)

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol APOA1