![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Cross-linking analysis of fmHDL apolipoprotein. fmHDL (apo A-I), fmHDL (apo A-I-SH), and fmHDL (no apo A-I) were cross-linked with BS3. The apolipoprotein cross-link products were resolved on a 4-20% SDS PAGE gel and visualized by apo A-I immunoblotting. An oligomer ladder was prepared with lipid-free apo A-I (apo A-I lane) for cross-link product identification. fmHDL migrated on the PAGE gel as a clearly visible band and were detectible by apo A-I immunoblotting (marked as fmHDL on the left). Image collected and cropped by CiteAb from the following publication (//www.jlr.org/lookup/doi/10.1194/jlr.M054635) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NB400-147-img0004.jpg "Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Cross-linking analysis of fmHDL apolipoprotein. fmHDL (apo A-I), fmHDL (apo A-I-SH), and fmHDL (no apo A-I) were cross-linked with BS3. The apolipoprotein cross-link products were resolved on a 4-20% SDS PAGE gel and visualized by apo A-I immunoblotting. An oligomer ladder was prepared with lipid-free apo A-I (apo A-I lane) for cross-link product identification. fmHDL migrated on the PAGE gel as a clearly visible band and were detectible by apo A-I immunoblotting (marked as fmHDL on the left). Image collected and cropped by CiteAb from the following publication (//www.jlr.org/lookup/doi/10.1194/jlr.M054635) licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP, PAGE |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Description | Goat Anti-Apolipoprotein A-I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. |
| Immunogen | Apolipoprotein A-I/ApoA1 was isolated from human plasma by density gradient centrifugation followed by HPLC purification, followed by repeated immunizations in goat. (Uniprot: P02647) |
| Localization | Secreted |
| Specificity | Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | APOA1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product have been tested by Western blot and IHC and are suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation, immunohistochemistry and for western blotting for highly sensitive qualitative analysis. Use in Sandwich-ELISA reported in scientific literature (PMID 26648662) |
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| Storage | Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles. |
| Buffer | 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - anti-APOA1 antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Immunohistochemistry-NB400-147-img0002.jpg "Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - anti-APOA1 antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.")
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Coomassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using anti-Human apolipoprotein A-I antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NB400-147-img0003.jpg "Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Coomassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using anti-Human apolipoprotein A-I antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.")
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