![Western Blot: Annexin V Antibody (M017) [NBP3-23207] - Western blot of human PC3 cells (lane 1), human breast tissue (lane 2), lung tissue (lane 3), skin tissue (lane 4), and brain tissue (lane 5). The blot was probed with mouse monoclonal anti-Annexin V antibody (NBP3-23207) at 1:500 (lanes 1-5).](http://images.novusbio.com/fullsize/nbp3-23207_mouse-annexin-v-mab-m017-19920231759444.jpg "Western Blot: Annexin V Antibody (M017) [NBP3-23207] - Western blot of human PC3 cells (lane 1), human breast tissue (lane 2), lung tissue (lane 3), skin tissue (lane 4), and brain tissue (lane 5). The blot was probed with mouse monoclonal anti-Annexin V antibody (NBP3-23207) at 1:500 (lanes 1-5).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clone | M017 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Clone M017 was generated from a proprietary antigen related to human Annexin V in the MDA-MB-231 breast cancer cell line. [UniProt# P08758] |
| Specificity | Clone M017 detects a 35 kDa protein corresponding to the molecular mass of Annexin V on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, and PC3 cells, as well as human skin, breast, lung, and brain. Mass spectrometry analysis of immunoprecipitates using NBP3-23207 in human MDA-MB-231 cell lysates confirms this antibody only detects Annexin A5 protein. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ANXA5 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Annexin V Antibody (NBP3-23207)Find related products by research area.
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| Gene Symbol | ANXA5 |
![Western Blot: Annexin V Antibody (M017) [NBP3-23207] - Western blot of A431 cell lysate only (lane 1), NBP3-23207 antibody only (lane 2), and NBP3-23207 antibody immunoprecipitate from A431 (lane 3). The blot was probed with mouse monoclonal anti-Annexin V antibody (NBP3-23207) at 1:500 (lanes 1-3). The asterisk shows the antibody heavy chain in immunopreciptates at 50 kDa, while Annexin V band is observed at 35 kDa.](http://images.novusbio.com/fullsize/nbp3-23207_mouse-annexin-v-mab-m017-19920231410119.jpg "Western Blot: Annexin V Antibody (M017) [NBP3-23207] - Western blot of A431 cell lysate only (lane 1), NBP3-23207 antibody only (lane 2), and NBP3-23207 antibody immunoprecipitate from A431 (lane 3). The blot was probed with mouse monoclonal anti-Annexin V antibody (NBP3-23207) at 1:500 (lanes 1-3). The asterisk shows the antibody heavy chain in immunopreciptates at 50 kDa, while Annexin V band is observed at 35 kDa.")
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