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Aminopeptidase N/CD13 Antibody [Biotin]

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U937 human histiocytic lymphoma cell line was stained with Sheep Anti-Human Aminopeptidase N/CD13 Bio-tinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3815, filled histogram) or control antibody (Catalog # BAF020, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.
U937 human histiocytic lymphoma cell line was stained with Sheep Anti-Human Aminopeptidase N/CD13 Bio-tinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3815, filled histogram) or control antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF3815
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF3815
Carrier Free
AF3815
Carrier Free
AF3815-SP
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Aminopeptidase N/CD13 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Aminopeptidase N/CD13
Lys69-Lys967
Accession # NP_001141
Specificity
Detects Aminopeptidase N/CD13 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse APN is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ANPEP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aminopeptidase N/CD13 Antibody [Biotin]

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Background

The human ANPEP gene encodes aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).

  1. Olsen, J. et al. (1988) FEBS Lett. 238:307.
  2. Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
  3. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ANPEP
Uniprot