![Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot alpha-1A Adrenergic R/ADRA1A protein expression. Nitrergic neuron alpha 1-, F(5, 12)=10.51, p=.0005 protein profiles are depicted in Panels 3A; GABAergic neuron alpha 1-, F(5, 12)=5.52, p=.007 protein profiles are presented in Panels 3D. Data show mean normalized protein O.D. measures+/-SEMfor the following treatment groups: Veh/Veh (n=6), MPP/Veh (n=6), PHTPP/Veh (n=6), Veh/NE (n=6), MPP/NE (n=6), and PHTPP/NE (n=6). *pSample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha-1A Adrenergic R/ADRA1A stained by alpha-1A Adrenergic R/ADRA1A antibody (NB100-78585) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nb100-78585_rabbit-polyclonal-alpha-1a-adrenergic-r-adra1a-antibody-910202420131298.jpg "Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot alpha-1A Adrenergic R/ADRA1A protein expression. Nitrergic neuron alpha 1-, F(5, 12)=10.51, p=.0005 protein profiles are depicted in Panels 3A; GABAergic neuron alpha 1-, F(5, 12)=5.52, p=.007 protein profiles are presented in Panels 3D. Data show mean normalized protein O.D. measures+/-SEMfor the following treatment groups: Veh/Veh (n=6), MPP/Veh (n=6), PHTPP/Veh (n=6), Veh/NE (n=6), MPP/NE (n=6), and PHTPP/NE (n=6). *pSample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha-1A Adrenergic R/ADRA1A stained by alpha-1A Adrenergic R/ADRA1A antibody (NB100-78585) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Gp, Pm, Rb, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha-1A Adrenergic R/ADRA1A. The exact sequence is proprietary. |
| Localization | Cell membrane |
| Predicted Species | Porcine (100%), Bovine (100%), Guinea Pig (100%), Rabbit (100%), Rhesus Macaque (100%), Monkey (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ADRA1A |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for alpha-1A Adrenergic R/ADRA1A Antibody (NB100-78585)Find related products by research area.
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