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5-HT3B Antibody (2H9H5) - BSA Free

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Western Blot: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis using HTR3B mAb against HEK293 (1) and HTR3B (AA: extra 23-150)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis of Hela cells using HTR3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Flow Cytometry: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis of Hela cells using HTR3B mouse mAb (green) and negative control (red).
Western Blot: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis using HTR3B mAb against human HTR3B (AA: extra 23-150) recombinant protein. (Expected MW is 41.1 kDa)
Western Blot: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis using HTR3B mouse mAb against SK-N-SH (1) and Hela (2) cell lysate.
ELISA: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
2H9H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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5-HT3B Antibody (2H9H5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human 5-HT3B (AA: extra 23-150) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HTR3B
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Western Blot 1:500-1:2000
Theoretical MW
50.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for 5-HT3B Antibody (2H9H5) - BSA Free

  • 5HT3B
  • 5-HT3B
  • 5-HT3-B
  • 5-HT3B5-hydroxytryptamine receptor 3B
  • 5-hydroxytryptamine (serotonin) receptor 3B
  • 5-hydroxytryptamine 3 receptor B subunit
  • HTR3B
  • Serotonin receptor 3B
  • serotonin-gated ion channel subunit

Background

The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit B of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It is not functional as a homomeric complex, but a pentaheteromeric complex with subunit A (HTR3A) displays the full functional features of this receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol HTR3B