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Recombinant Human 14-3-3 sigma/Stratifin Protein

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SDS-Page: Recombinant Human 14-3-3 sigma/Stratifin Protein [NBP1-30172] - 15% SDS-PAGE (3ug)
SDS-Page: Recombinant Human 14-3-3 sigma/Stratifin Protein [NBP1-30172] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human 14-3-3 sigma/Stratifin Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-248 of Human 14-3-3 sigma/Stratifin

Source: E.coli

Amino Acid Sequence: MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SFN
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
27.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 50 mM NaCl, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human 14-3-3 sigma/Stratifin Protein

  • 14-3-3 protein sigma
  • 1433 sigma
  • 14-3-3 sigma
  • Epithelial cell marker protein 1
  • HME1
  • SFN
  • stratifin
  • YWHAS

Background

14-3-3 sigma ,also known as Stratifin (SFN), belong to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Recombinant human 14-3-3 sigma was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human 14-3-3 sigma/Stratifin Protein
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Bioinformatics

Gene Symbol SFN