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14-3-3 beta Antibody

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Western blot shows lysates of TF-1 human erythroleukemic cell line cells, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat 14-3-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4724) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recom-binant human 14-3-3 beta, epsilon, eta, gamma, sigma, theta, and zeta (20 ng/lane) were included. A specific band for 14-3-3 beta was detected at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of TF-1 human erythroleukemic cell line cells, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat 14-3-3 beta Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4724-SP
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14-3-3 beta Antibody Summary

Immunogen
E. coli-derived recombinant human 14-3-3 beta
Met1-Asn246
Accession # P31946
Specificity
Detects human, mouse, and rat 14-3-3 beta in Western blots. Does not cross-react with other recombinant human 14-3-3 isoforms.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
YWHAB
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 14-3-3 beta Antibody

  • 14-3-3 alpha
  • 1433 beta
  • 14-3-3 beta
  • 14-3-3 protein beta/alpha
  • GW128
  • HS1
  • YWHAB

Background

14-3-3 proteins are a highly conserved family of homo- and heterodimeric phosphoserine/threonine-binding proteins present in all eukaryotic cells. 14-3-3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least 7 distinct mammalian 14-3-3 genes - alpha / beta , epsilon , eta , gamma , tau / theta , sigma , and zeta /δ.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol YWHAB
Uniprot