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![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Analysis in human cerebral cortex and pancreas tissues. Corresponding YWHAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Analysis in human cerebral cortex and pancreas tissues. Corresponding YWHAB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: 14-3-3 beta/alpha Antibody [NBP1-80611] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-80611-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cell line A-431 shows positivity in cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80611-img0003.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0010.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-80611] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-80611-img0004.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0007.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-80611] - Staining of human skin shows moderate to strong cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-80611-img0009.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0009.jpg "Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against HEK293 (1) and YWHAB (AA: 1-246)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of HepG2 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0005.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded endometrial cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mAb against human YWHAB (AA: 1-246) recombinant protein. (Expected MW is 54 kDa)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0008.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis using YWHAB mouse mAb against Hela (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Western-Blot-NBP2-61780-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of GC-7901 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunocytochemistry-Immunofluorescence-NBP2-61780-img0004.jpg)
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of paraffin-embedded rectum cancer tissues using YWHAB mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-Immunohistochemistry-NBP2-61780-img0006.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0001.jpg)
![ELISA: 14-3-3 beta/alpha Antibody (5B5G10) [NBP2-61780] - Analysis of Hela cells using YWHAB mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-5B5G10-ELISA-NBP2-61780-img0002.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: HepG2](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-SD0837-Western-Blot-NBP2-67833-img0009.jpg "Western Blot: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: HepG2")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Staining 14-3-3 alpha+beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-SD0837-Immunocytochemistry-Immunofluorescence-NBP2-67833-img0004.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-SD0837-Immunohistochemistry-Paraffin-NBP2-67833-img0008.jpg)
![Flow Cytometry: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of Hela cells with 14-3-3 alpha+beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-SD0837-Flow-Cytometry-NBP2-67833-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Staining 14-3-3 alpha+beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-SD0837-Immunocytochemistry-Immunofluorescence-NBP2-67833-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Staining 14-3-3 alpha+beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-SD0837-Immunocytochemistry-Immunofluorescence-NBP2-67833-img0003.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-SD0837-Immunohistochemistry-Paraffin-NBP2-67833-img0005.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of paraffin-embedded human breast tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-SD0837-Immunohistochemistry-Paraffin-NBP2-67833-img0006.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (SD0837) [NBP2-67833] - Analysis of paraffin-embedded mouse brain tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-SD0837-Immunohistochemistry-Paraffin-NBP2-67833-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-33454-img0008.jpg "Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 beta antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33454-img0003.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Rat cervix. 14-3-3 beta antibody [N1C2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0007.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-33454-img0005.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NBP1-33454] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 beta antibody [N1C2] diluted at 1:10000.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NBP1-33454-img0006.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody [NBP1-33454] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-33454-img0004.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-Western-Blot-NB100-665-img0001.jpg "Western Blot: 14-3-3 beta/alpha Antibody [NB100-665]")
![Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202411351616.jpg "Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Staining of PC-3 cell with 14-3-3 beta/alpha Antibody (6F6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202412493639.jpg)
![Flow Cytometry: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Overlay Peak curve showing Hela cells stained with 14-3-3 beta/alpha Antibody (6F6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202414183655.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (S08-4A7) [NBP3-14906] - Western blot detection of 14-3-3 beta/alpha in Hela, A549, MCF-7, U251, C2C12 cell lysates using 14-3-3 beta/alpha Rabbit mAb (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-S08-4A7-Western-Blot-NBP3-14906-img0001.jpg "Western Blot: 14-3-3 beta/alpha Antibody (S08-4A7) [NBP3-14906] - Western blot detection of 14-3-3 beta/alpha in Hela, A549, MCF-7, U251, C2C12 cell lysates using 14-3-3 beta/alpha Rabbit mAb (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.")
![Immunohistochemistry: 14-3-3 beta/alpha Antibody (S08-4A7) [NBP3-14906] - Immunohistochemical of 14-3-3 beta/alpha in Human tonsil tissue using 14-3-3 beta/alpha antibody at dilution 1/50](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-S08-4A7-Immunohistochemistry-NBP3-14906-img0002.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (S07-1A8) [NBP3-14905] - Western blot detection of 41701 in K562, Rat Brain, C6, 3T3, Hela cell lysates using 14-3-3 beta/alpha Rabbit mAb (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-S07-1A8-Western-Blot-NBP3-14905-img0001.jpg "Western Blot: 14-3-3 beta/alpha Antibody (S07-1A8) [NBP3-14905] - Western blot detection of 41701 in K562, Rat Brain, C6, 3T3, Hela cell lysates using 14-3-3 beta/alpha Rabbit mAb (1:1000 diluted). Predicted band size: 28kDa. Observed band size: 28kDa.")
![Immunohistochemistry-Paraffin: 14-3-3 beta/alpha Antibody (S07-1A8) [NBP3-14905] - Immunohistochemistry of 14-3-3 beta/alpha in paraffin-embedded Human tonsil using 14-3-3 beta/alpha Rabbit mAb at dilution 1/100](http://images.novusbio.com/fullsize/14-3-3-beta-alpha-Antibody-S07-1A8-Immunohistochemistry-Paraffin-NBP3-14905-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta Antibody (03) [NBP3-06516] - Staining of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/14-3-3-beta-Antibody-03-Immunocytochemistry-Immunofluorescence-NBP3-06516-img0001.jpg "Immunocytochemistry/Immunofluorescence: 14-3-3 beta Antibody (03) [NBP3-06516] - Staining of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
![Flow Cytometry: 14-3-3 beta Antibody (03) [NBP3-06516] - Analysis of Human YWHAB expression on Molt-4 cells. The cells were treated, stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/14-3-3-beta-Antibody-03-Flow-Cytometry-NBP3-06516-img0002.jpg)
![Western Blot: 14-3-3 beta Antibody (05) [NBP3-06157] - Anti-YWHAB mouse monoclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 115000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:32 kDa](http://images.novusbio.com/fullsize/14-3-3-beta-Antibody-05-Western-Blot-NBP3-06157-img0001.jpg "Western Blot: 14-3-3 beta Antibody (05) [NBP3-06157] - Anti-YWHAB mouse monoclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Mouse IgG H&L (Dylight800) at 115000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:32 kDa")
![14-3-3 beta Antibody (05) [NBP3-06157] - cynoYWHAB was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate Lane B: 0.5 mg Hela Whole Cell Lysate 2 uL anti-cyno YWHAB mouse monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody:Anti-cyno YWHAB mouse monoclonal antibody, at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 32 kDa Observed band size: 32 kDa](http://images.novusbio.com/fullsize/14-3-3-beta-Antibody-05-NBP3-06157-img0002.jpg)
![Western Blot: 14-3-3 beta Antibody [NBP2-98453] - Anti-14-3-3 beta rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: NCI-H1299 Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/14-3-3-beta-Antibody-Western-Blot-NBP2-98453-img0003.jpg "Western Blot: 14-3-3 beta Antibody [NBP2-98453] - Anti-14-3-3 beta rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: NCI-H1299 Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 32 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 14-3-3 beta Antibody [NBP2-98453] - 14-3-3 beta was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 4 ul anti-14-3-3 beta rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 beta rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa](http://images.novusbio.com/fullsize/14-3-3-beta-Antibody-Immunoprecipitation-NBP2-98453-img0001.jpg)