PTGER2 Antibodies
We offer PTGER2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each PTGER2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PTGER2 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PTGER2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PTGER2 polyclonal antibodies and browse our PTGER2 monoclonal antibody catalog.
Alternate Names for PTGER2 Antibodies
anti-PTGER2 antibody, anti-EP2 antibody, anti-PGE receptor EP2 subtype antibody, anti-PGE2 receptor EP2 subtype antibody, anti-prostaglandin E receptor 2 (subtype EP2), 53kD antibody, anti-prostaglandin E receptor 2 (subtype EP2), 53kDa antibody, anti-prostaglandin E2 receptor EP2 subtype antibody, anti-Prostanoid EP2 receptor antibody
8 Results for "ptger2" in Primary Antibodies
![Western Blot: PTGER2 Antibody (2G10L6) [NBP3-16740] - Analysis of extracts of various cell lines, using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Western-Blot-NBP3-16740-img0005.jpg "Western Blot: PTGER2 Antibody (2G10L6) [NBP3-16740] - Analysis of extracts of various cell lines, using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: PTGER2 Antibody (2G10L6) [NBP3-16740] - Immunofluorescence analysis of U-2 OS cells using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Immunocytochemistry-Immunofluorescence-NBP3-16740-img0004.jpg)
![Immunohistochemistry-Paraffin: PTGER2 Antibody (2G10L6) [NBP3-16740] - Human placenta using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Immunohistochemistry-Paraffin-NBP3-16740-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PTGER2 Antibody (2G10L6) [NBP3-16740] - Immunofluorescence analysis of C6 cells using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Immunocytochemistry-Immunofluorescence-NBP3-16740-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PTGER2 Antibody (2G10L6) [NBP3-16740] - Immunofluorescence analysis of NIH-3T3 cells using PGE Receptor EP2 (PGE Receptor EP2 (PTGER2)) Rabbit mAb (NBP3-16740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTGER2-Antibody-2G10L6-Immunocytochemistry-Immunofluorescence-NBP3-16740-img0003.jpg)
![Western Blot: PTGER2 Antibody (JE30-72) [NBP3-32874] - Western blot analysis of PTGER2 on different lysates with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/1,000 dilution. Lane 1: JAR cell lysate (15 ug/Lane) Lane 2: NIH/3T3 cell lysate (15 ug/Lane) Lane 3: Human placenta tissue lysate (30 ug/Lane) Lane 4: Mouse placenta tissue lysate (30 ug/Lane) Lane 5: Mouse lung tissue lysate (30 ug/Lane) Lane 6: Rat lung tissue lysate (30 ug/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32874) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32874_rabbit-ptger2-mab-je30-72-288202412442965.jpg "Western Blot: PTGER2 Antibody (JE30-72) [NBP3-32874] - Western blot analysis of PTGER2 on different lysates with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/1,000 dilution. Lane 1: JAR cell lysate (15 ug/Lane) Lane 2: NIH/3T3 cell lysate (15 ug/Lane) Lane 3: Human placenta tissue lysate (30 ug/Lane) Lane 4: Mouse placenta tissue lysate (30 ug/Lane) Lane 5: Mouse lung tissue lysate (30 ug/Lane) Lane 6: Rat lung tissue lysate (30 ug/Lane) Predicted band size: 40 kDa Observed band size: 50 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32874) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTGER2 Antibody (JE30-72) [NBP3-32874] - Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32874) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32874_rabbit-ptger2-mab-je30-72-298202414262611.jpg)
![Immunocytochemistry/ Immunofluorescence: PTGER2 Antibody (JE30-72) [NBP3-32874] - Immunocytochemistry analysis of JAR cells labeling PTGER2 with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PTGER2 antibody (NBP3-32874) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32874_rabbit-ptger2-mab-je30-72-29202410484830.jpg)
![Flow Cytometry: PTGER2 Antibody (JE30-72) [NBP3-32874] - Flow cytometric analysis of JAR cells labeling PTGER2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32874, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32874_rabbit-ptger2-mab-je30-72-29202416453292.jpg)
![Immunohistochemistry-Paraffin: PTGER2 Antibody - BSA Free [NBP1-02321] - Staining of human kidney formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.](http://images.novusbio.com/fullsize/PTGER2-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-02321-img0003.jpg "Immunohistochemistry-Paraffin: PTGER2 Antibody - BSA Free [NBP1-02321] - Staining of human kidney formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.")
![Immunohistochemistry-Paraffin: PTGER2 Antibody [NLS971] - Anti-PTGER2 / EP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTGER2-Antibody-Immunohistochemistry-Paraffin-NLS971-img0003.jpg "Immunohistochemistry-Paraffin: PTGER2 Antibody [NLS971] - Anti-PTGER2 / EP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: PTGER2 Antibody [NLS971] - IHC analysis of Ovary, Epithelium using NLS971 at 6 ug/ ml.](http://images.novusbio.com/fullsize/PTGER2-Antibody-Immunohistochemistry-Paraffin-NLS971-img0001.jpg)
![Immunohistochemistry-Paraffin: PTGER2 Antibody [NLS971] - Analysis of anti-EP2 antibody with human lung.](http://images.novusbio.com/fullsize/PTGER2-Antibody-Immunohistochemistry-Paraffin-NLS971-img0002.jpg)
![Western Blot: PTGER2 Antibody (SR1927) [NBP3-21756] - Western blot analysis using NBP3-21756 in JAR cell lysate.](http://images.novusbio.com/fullsize/nbp3-21756_rabbit-ptger2-mab-sr1927-57202317161828.jpg "Western Blot: PTGER2 Antibody (SR1927) [NBP3-21756] - Western blot analysis using NBP3-21756 in JAR cell lysate.")
![Immunohistochemistry-Paraffin: PTGER2 Antibody - BSA Free [NBP1-02375] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.](http://images.novusbio.com/fullsize/PTGER2-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-02375-img0002.jpg "Immunohistochemistry-Paraffin: PTGER2 Antibody - BSA Free [NBP1-02375] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 40 ug/ml.")
![Immunohistochemistry: PTGER2 Antibody (8D8) [NBP3-15217] - IHC image of PTGER2 antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PTGER2-Antibody-8D8-Immunohistochemistry-NBP3-15217-img0001.jpg "Immunohistochemistry: PTGER2 Antibody (8D8) [NBP3-15217] - IHC image of PTGER2 antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PTGER2 Antibody (8D8) [NBP3-15217] - IHC image of PTGER2 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PTGER2-Antibody-8D8-Immunohistochemistry-NBP3-15217-img0002.jpg)
