POMC Antibodies
We offer POMC Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Peptide ELISA, Sandwich ELISA, Western Blot. Each POMC Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our POMC Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the POMC Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our POMC polyclonal antibodies and browse our POMC monoclonal antibody catalog.
Alternate Names for POMC Antibodies
anti-POMC antibody, anti-ACTH antibody, anti-adrenocorticotropic hormone antibody, anti-adrenocorticotropin antibody, anti-alpha-melanocyte-stimulating hormone antibody, anti-alpha-MSH antibody, anti-beta-endorphin antibody, anti-beta-LPH antibody, anti-beta-melanocyte-stimulating hormone antibody, anti-beta-MSH antibody, anti-CLIP antibody, anti-corticotropin-like intermediary peptide antibody, anti-corticotropin-lipotropin antibody, anti-gamma-LPH antibody, anti-gamma-MSH antibody, anti-lipotropin beta antibody, anti-lipotropin gamma antibody, anti-LPH antibody, anti-melanotropin alpha antibody, anti-melanotropin beta antibody, anti-melanotropin gamma antibody, anti-met-enkephalin antibody, anti-MSH antibody, anti-NPP antibody, anti-POC antibody, anti-pro-ACTH-endorphin antibody, anti-pro-opiomelanocortin antibody, anti-proopiomelanocortin antibody, anti-proopiomelanocortin preproprotein antibody
12 Results for "pomc" in Primary Antibodies
![Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-NB100-1533-img0008.jpg "Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.")
![Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NB100-1533-img0003.jpg)
![Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-NB100-1533-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: POMC Antibody [NB100-1533] - Pathogenic POMC-C28F mutation is completely rescued by an intragenic suppressor mutation C50S.(A) Schematic diagram showing the sequence & positions of Cys residues in POMC-WT, C28F, & C28F/C50S. (B) Western blot analyses of POMC-Flag immunoprecipitates in HEK293T cells transfected with a combination of Myc- or Flag-tagged POMC under nonreducing (– beta -ME) & reducing (+ beta -ME) SDS-PAGE. Two panels were from the same experiment at the same exposure time, with the irrelevant lanes in the middle cut off. (C) Sucrose gradient fractionation & Western blot analysis of HEK293T cells expressing POMC-C28F or -C28F/C50S under nonreducing (– beta -ME) & reducing (+ beta -ME) SDS-PAGE. (D) Metabolic labeling experiments to visualize the maturation of nascent POMC in HEK293T cells transfected with POMC-Myc. Cells were pulsed for 30 minutes & chased for the indicated times, followed by immunoprecipitation with anti-Myc agarose beads & separation on SDS-PAGE gels under nonreducing or reducing conditions & autoradiography. (E) Representative confocal images of POMC in POMC-transfected WT N2a cells. White arrows point to secreted POMC in granules, while yellow arrows point to perinuclear POMC, possibly in the form of aggregates. KDEL marks the ER. Representative data from at least 2 independent experiments shown. Image collected & cropped by CiteAb from the following publication (//www.jci.org/articles/view/96420), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1533_goat-polyclonal-pomc-antibody-310202415535174.jpg)
![Western Blot: POMC Antibody (2B2) [NBP2-45369] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POMC.](http://images.novusbio.com/fullsize/POMC-Antibody-2B2-Western-Blot-NBP2-45369-img0005.jpg "Western Blot: POMC Antibody (2B2) [NBP2-45369] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POMC.")
![Immunohistochemistry: POMC Antibody (2B2) [NBP2-45369] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/POMC-Antibody-2B2-Immunohistochemistry-NBP2-45369-img0004.jpg)
![Immunohistochemistry: POMC Antibody (2B2) [NBP2-45369] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/POMC-Antibody-2B2-Immunohistochemistry-NBP2-45369-img0001.jpg)
![Immunohistochemistry: POMC Antibody (2B2) [NBP2-45369] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/POMC-Antibody-2B2-Immunohistochemistry-NBP2-45369-img0002.jpg)
![Immunohistochemistry: POMC Antibody (2B2) [NBP2-45369] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/POMC-Antibody-2B2-Immunohistochemistry-NBP2-45369-img0003.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NBP2-55008] - Staining of human pituitary gland.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-55008-img0009.jpg "Immunohistochemistry-Paraffin: POMC Antibody [NBP2-55008] - Staining of human pituitary gland.")
![Immunohistochemistry-Paraffin: POMC Antibody [NBP2-55008] - Staining of human testis.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-55008-img0004.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NBP2-55008] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-55008-img0005.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NBP2-55008] - Staining of human liver.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-55008-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: POMC Antibody [NBP2-55008] - Staining of human cerebral cortex, liver, pituitary gland and testis using Anti-POMC antibody NBP2-55008 (A) shows similar protein distribution across tissues to independent antibody NBP2-57719 (B).](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-55008-img0008.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NBP2-57719] - Staining of human pituitary gland.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-57719-img0009.jpg "Immunohistochemistry-Paraffin: POMC Antibody [NBP2-57719] - Staining of human pituitary gland.")
![Immunohistochemistry-Paraffin: POMC Antibody [NBP2-57719] - Staining of human testis.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-57719-img0004.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NBP2-57719] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-57719-img0005.jpg)
![Immunohistochemistry-Paraffin: POMC Antibody [NBP2-57719] - Staining of human liver.](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-57719-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: POMC Antibody [NBP2-57719] - Staining of human cerebral cortex, liver, pituitary gland and testis using Anti-POMC antibody NBP2-57719 (A) shows similar protein distribution across tissues to independent antibody NBP2-55008 (B).](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP2-57719-img0008.jpg)
![Western Blot: POMC Antibody (6D2B5) [NBP2-61793] - Analysis using POMC mAb against human POMC (AA: 1-150) recombinant protein. (Expected MW is 41.9 kDa)](http://images.novusbio.com/fullsize/POMC-Antibody-6D2B5-Western-Blot-NBP2-61793-img0003.jpg "Western Blot: POMC Antibody (6D2B5) [NBP2-61793] - Analysis using POMC mAb against human POMC (AA: 1-150) recombinant protein. (Expected MW is 41.9 kDa)")
![Immunocytochemistry/Immunofluorescence: POMC Antibody (6D2B5) [NBP2-61793] - Analysis of Hela cells using POMC mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/POMC-Antibody-6D2B5-Immunocytochemistry-Immunofluorescence-NBP2-61793-img0002.jpg)
![Western Blot: POMC Antibody (6D2B5) [NBP2-61793] - Analysis using POMC mAb against HEK293 (1) and POMC (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/POMC-Antibody-6D2B5-Western-Blot-NBP2-61793-img0004.jpg)
![ELISA: POMC Antibody (6D2B5) [NBP2-61793] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/POMC-Antibody-6D2B5-ELISA-NBP2-61793-img0001.jpg)
![Western Blot: POMC Antibody (JE32-88) [NBP3-32842] - Western blot analysis of POMC on rat pituitary tissue lysates with Rabbit anti-POMC antibody (NBP3-32842) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32842) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32842_rabbit-pomc-mab-je32-88-28820241246160.jpg "Western Blot: POMC Antibody (JE32-88) [NBP3-32842] - Western blot analysis of POMC on rat pituitary tissue lysates with Rabbit anti-POMC antibody (NBP3-32842) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32842) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: POMC Antibody (JE32-88) [NBP3-32842] - Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-POMC antibody (NBP3-32842) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32842) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32842_rabbit-pomc-mab-je32-88-29820241492661.jpg)
![Western Blot: POMC Antibody (SR1489) [NBP3-21845] - Western blot analysis using NBP3-21845 in mouse pituitary lysate.](http://images.novusbio.com/fullsize/nbp3-21845_rabbit-pomc-mab-sr1489-57202316112140.jpg "Western Blot: POMC Antibody (SR1489) [NBP3-21845] - Western blot analysis using NBP3-21845 in mouse pituitary lysate.")
![Western Blot: POMC Antibody (3E11) [H00005443-M01] - Analysis of POMC expression in transfected 293T cell line by POMC monoclonal antibody (M01), clone 3E11.Lane 1: POMC transfected lysate(29.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/POMC-Antibody-3E11-Western-Blot-H00005443-M01-img0003.jpg "Western Blot: POMC Antibody (3E11) [H00005443-M01] - Analysis of POMC expression in transfected 293T cell line by POMC monoclonal antibody (M01), clone 3E11.Lane 1: POMC transfected lysate(29.4 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: POMC Antibody (3E11) [H00005443-M01] - Detection limit for recombinant GST tagged POMC is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/POMC-Antibody-3E11-Sandwich-ELISA-H00005443-M01-img0002.jpg)
![Immunohistochemistry: POMC Antibody (PD01-40) [NBP3-32841] - Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-POMC antibody (NBP3-32841) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32841) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32841_mouse-pomc-mab-pd01-40-29820241358553.jpg "Immunohistochemistry: POMC Antibody (PD01-40) [NBP3-32841] - Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-POMC antibody (NBP3-32841) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32841) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: POMC Antibody [NBP3-12106] - Formalin-Fixed, Paraffin-Embedded (FFPE) HIER of Human pituitary, using 10 mM sodium citrate buffer pH 6.0](http://images.novusbio.com/fullsize/POMC-Antibody-Immunohistochemistry-Paraffin-NBP3-12106-img0001.jpg "Immunohistochemistry-Paraffin: POMC Antibody [NBP3-12106] - Formalin-Fixed, Paraffin-Embedded (FFPE) HIER of Human pituitary, using 10 mM sodium citrate buffer pH 6.0")