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POMC Antibody (967905) [Unconjugated]

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Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human POMC Monoclonal Antibody (Catalog # MAB9498) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for POMC at approximately 10-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human POMC Monoclonal Antibody (Catalog # MAB9498) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
POMC was detected in immersion fixed paraffin-embedded sections of human pituitary using Mouse Anti-Human POMC Monoclonal Antibody (Catalog # MAB9498) at 5 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
967905
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB9498-SP
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POMC Antibody (967905) [Unconjugated] Summary

Immunogen
Human Adrenocorticotropic hormone/ACTH) synthetic peptide
Accession # P01189
Specificity
Detects human POMC in immunohistochemistry.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for POMC Antibody (967905) [Unconjugated]

  • ACTH
  • adrenocorticotropic hormone
  • adrenocorticotropin
  • alpha-melanocyte-stimulating hormone
  • alpha-MSH
  • beta-endorphin
  • beta-LPH
  • beta-melanocyte-stimulating hormone
  • beta-MSH
  • CLIP
  • corticotropin-like intermediary peptide
  • corticotropin-lipotropin
  • gamma-LPH
  • gamma-MSH
  • lipotropin beta
  • lipotropin gamma
  • LPH
  • melanotropin alpha
  • melanotropin beta
  • melanotropin gamma
  • met-enkephalin
  • MSH
  • NPP
  • POC
  • POMC
  • pro-ACTH-endorphin
  • proopiomelanocortin preproprotein
  • proopiomelanocortin
  • pro-opiomelanocortin

Background

Adrenocorticotropic hormone (ACTH)/corticotropin is a 39 amino acid peptide generated by cleavage of the POMC, a preprotein which also can also be cleaved to produce MSH, Beta-endorphin and Met-enkephalin, depending on tissue expression and context. ACTH is a ligand for the ACTH receptor, also known as melanocortin receptor 2. ACTH is secreted by the anterior pituitary gland upon stimulation by corticotropin-releasing hormone (CRH) which is secreted by the hypothalamus. The principal effect of ACTH is production of cortisol by the adrenal gland but it also mediates bone formation and osteoblast survival. ACTH has been shown to induce insulin resistance in adipocytes and promote pro-inflammatory adipokine secretion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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POMC Antibody
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MAB9498
POMC Antibody (967905) [Unconjugated]
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