Ly-6G/Ly-6C Antibodies
Ly-6G/Ly-6C (Lymphocyte antigen 6 complex locus G/C), are both part of the Ly-6 family of proteins that are expressed on myeloid cells and plays a role in both bone marrow development and the differentiation and maturation of granulocytes and neutrophils. The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein, and with Ly-6Chi, a 14 kD protein, whereas the 1A8 antibody is specific for Ly-6G. We offer antibodies to detect Ly-6G/Ly-6C that have been cited in 25+ publications. Browse our catalog of monoclonal and Ly6G/Ly6C-conjugated antibodies that are suitable in mouse. Validated research applications for Ly-6G/Ly-6C antibodies include CyTOF-ready, Flow Cytometry, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, IP, and Western Blot. Choose the anti-Ly6G/Ly6C antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products. Each Ly6G antibody is fully covered by our Guarantee+.
Alternate Names for Ly-6G/Ly-6C Antibodies
anti-Gr-1 antibody, anti-Gr1 antibody, anti-Ly-6G antibody
8 Results for "ly-6g-ly-6c" in Primary Antibodies
![Western Blot: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Mice were treated with oil in combination with injection of lenti-NC (NC, n = 10), CCl4 in combination with injection of lenti-NC (NC + CCl4, n = 10), oil in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA, n = 10), and CCl4 in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA + CCl4, n = 10). b The protein level of F4/80, CD11b and LY6C was determined by western blot. GAPDH was used as an internal control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2323-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Western-Blot-NBP2-00441-img0011.jpg "Western Blot: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Mice were treated with oil in combination with injection of lenti-NC (NC, n = 10), CCl4 in combination with injection of lenti-NC (NC + CCl4, n = 10), oil in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA, n = 10), and CCl4 in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA + CCl4, n = 10). b The protein level of F4/80, CD11b and LY6C was determined by western blot. GAPDH was used as an internal control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2323-5), licensed under a CC-BY license.")
![Immunohistochemistry: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Mice were treated with oil in combination with injection of lenti-NC (NC, n = 10), CCl4 in combination with injection of lenti-NC (NC + CCl4, n = 10), oil in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA, n = 10), and CCl4 in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA + CCl4, n = 10). a Immunohistochemistry analysis was performed to detect the expression of F4/80 and LY6C; scale bar = 400 um for 10 x and 100 um for 40x. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2323-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Immunohistochemistry-NBP2-00441-img0012.jpg)
![Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - 1 ug/1^6 BALB/c bone marrow cells were stained with either rat IgG2b: FITC (as an isotype control) or rat anti-mouse Ly-6G: FITC. Large cells were then gated and analyzed on a flow cytometer.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Flow-Cytometry-NBP2-00441-img0002.jpg)
![Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Analysis using the Allophycocyanin conjugate of NBP2-00441. Staining of C57BL/6 bone marrow cells with 0.125 ug of Rat IgG2b kappa Isotype Control APC (blue histogram) or 0.125 ug of Anti-Mouse Ly-6G (Gr-1) APC (purple histogram).](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Flow-Cytometry-NBP2-00441-img0008.jpg)
![Immunohistochemistry-Paraffin: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Analysis of a FFPE tissue section of mouse bone marrow using 1:100 dilution of Lot E04586-1632 of Ly-6G antibody (clone RB6-8C5). The antibody generated specific staining in a subset of cells in the tested section.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Immunohistochemistry-Paraffin-NBP2-00441-img0007.jpg)
![Immunohistochemistry-Paraffin: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Analysis of a FFPE tissue section of mouse bone marrow using 1:200 dilution of Lot A-1 of Ly-6G antibody (clone RB6-8C5). The antibody generated specific staining in a subset of cells in the tested section. The neutrophils (identifiable from typical nuclear morphology) showed stronger signal than the neighboring cells.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Immunohistochemistry-Paraffin-NBP2-00441-img0006.jpg)
![Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Staining of mouse bone marrow with Anti-Mouse Ly-6G (Gr-1) FITC and Anti-Human/Mouse CD45R (B220) PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Flow-Cytometry-NBP2-00441-img0001.jpg)
![Immunohistochemistry: Ly-6G/Ly-6C Antibody (RB6-8C5) - BSA Free [NBP2-00441] - Silencing lnc-Lfar1 alleviates CCl4-induced proinflammatory activation of macrophages.Mice were treated with oil in combination with injection of lenti-NC (NC, n = 10), CCl4 in combination with injection of lenti-NC (NC + CCl4, n = 10), oil in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA, n = 10), & CCl4 in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA + CCl4, n = 10). a Immunohistochemistry analysis was performed to detect the expression of F4/80 & LY6C; scale bar = 400 μm for 10 × and 100 μm for 40×. b The protein level of F4/80, CD11b & LY6C was determined by western blot. GAPDH was used as an internal control. c The mRNA level of F4/80, Ly6c, Ccr2, Cd20, Il-6, iNos, Ccl5, Cxcl5, Cxcl9 & Cxcl10 was determined by qRT-PCR. *p < 0.05 vs NC, #p < 0.05 vs NC + CCl4. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32071306), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-00441_rat-monoclonal-ly-6g-ly-6c-antibody-rb6-8c5-31020241612214.jpg)
![Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Histopathology of lung sections collected at 24 h pbi from mice co-infected with mouse adapted influenza A/Puerto Rico/8/34 (H1N1) (PR8) and type 2 pneumococcal strain D39 variants. Serial lung sections were subjected to immunohistochemistry (IHC) for pneumococcus using Rabbit Anti-Streptococcus pneumoniae Polyclonal Antibody (Catalog # NB100-64502) at 4 ug/mL or neutrophils using Rat Anti-Ly-6G/Ly-6C Monoclonal Antibody (Catalog # NB600-1387) at 2 ug/mL. Representative images at 4x magnification with 60x magnification inset are shown. Images courtesy of Dr. Amanda P. Smith, UTHSC, TN //doi.org/10.1101/659557](http://images.novusbio.com/fullsize/Ly-6G6C-Antibody-NIMP-R14-Immunohistochemistry-NB600-1387-img0006.jpg "Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Histopathology of lung sections collected at 24 h pbi from mice co-infected with mouse adapted influenza A/Puerto Rico/8/34 (H1N1) (PR8) and type 2 pneumococcal strain D39 variants. Serial lung sections were subjected to immunohistochemistry (IHC) for pneumococcus using Rabbit Anti-Streptococcus pneumoniae Polyclonal Antibody (Catalog # NB100-64502) at 4 ug/mL or neutrophils using Rat Anti-Ly-6G/Ly-6C Monoclonal Antibody (Catalog # NB600-1387) at 2 ug/mL. Representative images at 4x magnification with 60x magnification inset are shown. Images courtesy of Dr. Amanda P. Smith, UTHSC, TN //doi.org/10.1101/659557")
![Immunohistochemistry-Frozen: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Staining for Ly-6G/Ly-6C FITC of frozen section of mouse spleen.](http://images.novusbio.com/fullsize/Ly-6G6C-Antibody-NIMP-R14-Immunohistochemistry-Frozen-NB600-1387-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Ly-6G/Ly-6C Antibody (RB6-8C5) - Chimeric [NBP3-11978] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of RB6-8C5 (NBP3-11978) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11978, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11978-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ly-6G/Ly-6C Antibody (RB6-8C5) - Chimeric [NBP3-11978] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of RB6-8C5 (NBP3-11978) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11978, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) - Chimeric [NBP3-11978] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RB6-8C5 (NBP3-11978, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Chimeric-Flow-Cytometry-NBP3-11978-img0002.jpg)
![Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441PE] - Total BALB/c bone marrow cell suspension was stained with Anti-Mouse Ly-6G (Gr-1) PE. Viable cells were gated on lymphoid (R2) and myeloid (R3) populations based on their scatter. Restricted expression of Ly-6G by myeloid lineage (B) and not by lymphoid lineage (A) is demonstrated. Green histograms are autofluorescence of bone marrow cells.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Flow-Cytometry-NBP2-00441PE-img0001.jpg "Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441PE] - Total BALB/c bone marrow cell suspension was stained with Anti-Mouse Ly-6G (Gr-1) PE. Viable cells were gated on lymphoid (R2) and myeloid (R3) populations based on their scatter. Restricted expression of Ly-6G by myeloid lineage (B) and not by lymphoid lineage (A) is demonstrated. Green histograms are autofluorescence of bone marrow cells.")