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Calnexin Antibodies

We offer Calnexin Antibodies for use in common research applications: Electron Microscopy, ELISA, Enzyme Immunoassay, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each Calnexin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Calnexin Antibodies can be used in a variety of model species: Avian, Bovine, C. elegans, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Human, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Xenopus, Zebrafish. Use the list below to choose the Calnexin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Calnexin polyclonal antibodies and browse our Calnexin monoclonal antibody catalog.

Alternate Names for Calnexin Antibodies

anti-Calnexin antibody, anti-CANX antibody, anti-CNX antibody, anti-IP90FLJ26570 antibody, anti-Major histocompatibility complex class I antigen-binding protein p88 antibody, anti-P90 antibody

22 Results for "calnexin" in Primary Antibodies

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NB100-1965

Calnexin Antibody - BSA Free

(3 Reviews)

(45 Publications)

0.1 ml

NB100-1965

$Western Blot: Calnexin Antibody [NB100-1965] - Subcellular localization of TM6SF2.Immunoaffinity isolation of ER and Golgi complex from mouse liver. ER and Golgi fractions were prepared from mouse liver microsomes by immunoaffinity chromatography as described under Experimental Procedures. Microsome membranes were dissolved in RIPA buffer, and equal volumes were separated on 10% SDS-PAGE and immunoblotting as described under Experimental Procedures. BiP, binding immunoglobulin protein; Gos28, Golgi SNAP receptor complex member 1; *, nonspecific band. Image collected and cropped by Citeab from the following publication (Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem (2016) licensed under a CC-BY license.$

Immunohistochemistry: Calnexin Antibody [NB100-1965] - Calnexin immunostaining in the anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5-T2 (D.I.O. zebrafish followed by 3,5-T2), D.I.O. with 3,5-T2 (D.I.O. zebrafish treated with 3,5-T2). (A) AI of ctrl zebrafish (B) AI of D.I.O. (C) AI of D.I.O. flw 3,5-T2. (D) AI of D.I.O. with 3,5-T2. The arrows indicate calnexin immunoexpression in the enteroendocrine and goblet cells. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2076-2615/10/7/1131/htm#) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1965) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1965) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Calnexin Antibody (NB100-1965) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: Calnexin Antibody - BSA Free [NB100-1965] - Whole cell lysate or exosome from MDA-MD-231 cells was loaded with 20 ug/lane. 10% SDS-PAGE. Calnexin Antibody (NB100-1965) was used for primary antibody: 1:3000, 4C, overnight. Image from verified customer review.

Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on rat FR cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.

Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.

Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on Daudi cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on NIH3T3 cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: Calnexin Antibody - BSA Free [NB100-1965] - Hepatic apoB levels (A), liver function tests (B), & relative hepatic levels of selected transcripts encoding proteins involved in activation of fibrosis & ER stress (C) in male WT & Tm6sf2−/− mice described in Fig. 3A.A, immunoblotting analysis was performed on liver protein (50 μg) using a rabbit anti-mouse apoB polyclonal antibody (1:1,000; Abcam) & ECL (SuperSignal West Pico Kit, Thermo Scientific). The ECL signal was visualized using a LI-COR imager (Odyssey Fc imager) & analyzed using LI-COR Image Studio software. B, plasma levels of aspartate aminotransferase (AST) & alanine aminotransferase (ALT) in chow-fed male mice. C, RNA levels were detected using quantitative real-time PCR (qRT-PCR), normalized to levels of 36B4 & expressed relative to the levels in the WT animals (n = 5). COL1A1, collagen alpha-1(I) chain; ACTA2, actin, aortic smooth muscle; XBP1s/u, X-box-binding protein 1 spliced/unspliced; ATF4, activating transcription factor 4; EDEM, ER degradation enhancer, mannosidase alpha -like 1; CHOP(DDIT3), C/EBP-homologous protein. Values are means ± S.E. (error bars). *, p < 0.05. AU, arbitrary units. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27013658), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, Gp, Rb, Sh, Xp, Ze, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, Gp, Rb, Sh, Xp, Ze, Ca

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-1965AF350
Alexa Fluor 405	NB100-1965AF405
Alexa Fluor 488	NB100-1965AF488
Alexa Fluor 532	NB100-1965AF532
Alexa Fluor 594	NB100-1965AF594
Alexa Fluor 647	NB100-1965AF647
Alexa Fluor 700	NB100-1965AF700
Alexa Fluor 750	NB100-1965AF750
Allophycocyanin	NB100-1965APC
Biotin	NB100-1965B
CoraFluor 1	NB100-1965CL1
DyLight 350	NB100-1965UV
DyLight 405	NB100-1965V
DyLight 488	NB100-1965G
DyLight 550	NB100-1965R
DyLight 594	NB100-1965DL594
DyLight 650	NB100-1965C
DyLight 680	NB100-1965FR
DyLight 755	NB100-1965IR
FITC	NB100-1965F
HRP	NB100-1965H
Janelia Fluor 525	NB100-1965JF525
Janelia Fluor 549	NB100-1965JF549
Janelia Fluor 585	NB100-1965JF585
Janelia Fluor 635	NB100-1965JF635
Janelia Fluor 646	NB100-1965JF646
Janelia Fluor 669	NB100-1965JF669
PE	NB100-1965PE
PerCP	NB100-1965PCP
mFluor Violet 450 SE	NB100-1965MFV450
mFluor Violet 500 SE	NB100-1965MFV500
mFluor Violet 610 SE	NB100-1965MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-1965SS

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NB100-1974

Calnexin Antibody - BSA Free

(1 Review)

(12 Publications)

0.1 ml

NB100-1974

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB100-1974) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: Calnexin Antibody [NB100-1974] - Identification of plasma exosomes and differentially expressed exosomal miRNAs. Confirmation of the exosomes markers with Western blotting indicated the presence of CD63 but the absence of calnexin in exosomes. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fonc.2019.00459/full), licensed under a CC-BY license.

Immunohistochemistry: Calnexin Antibody [NB100-1974] - Analysis of Calnexin in mouse bladder using DAB with hematoxylin counterstain.

Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on rat FR cells with Calnexin Antibody NB100-1974 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: Calnexin Antibody [NB100-1974] - Analysis of Calnexin in HeLa whole cell lysate.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1974] - Zebrafish Vwf forms pseudo-Weibel-Palade bodies (pseudo-WPBs) in mammalian cell culture. pzVwf/Myc-HIS (zebrafish Vwf) plasmids were transfected into HEK293T cells. Anti-Myc antibody conjugated to Alexa Fluor 488 (green channel) was used for detection and anti-calnexin antibody conjugated to Alexa Fluor 594 (red channel) labeled endoplasmic reticulum (ER). Both constructs demonstrate formation of elongated Myc positive and ER negative structures (absence of yellow signal in the merged panels) characteristic of pseudo-WPBs. Scale bars, 2.5 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ah/2012/214209/), licensed under a CC-BY license.

Flow Cytometry: Calnexin Antibody [NB100-1974] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-1974. An intracellular stain was performed on Jurkat cells with Calnexin antibody NB100-1974AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange).

Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on HeLa cells with Calnexin antibody NB100-1974AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.

Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on Daudi cells with Calnexin Antibody NB100-1974 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on NIH3T3 cells with Calnexin Antibody NB100-1974 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Simple Western: Calnexin Antibody [NB100-1974] - Image shows a specific band for Calnexin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Calnexin was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-Calnexin Affinity Purified Polyclonal Antibody conjugated to FITC (Catalog # NB100-1974F) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody - BSA Free [NB100-1974] - Zebrafish Vwf forms pseudo-Weibel-Palade bodies (pseudo-WPBs) in mammalian cell culture. pVWF/Myc-HIS (human VWF, (a–c)) or pzVwf/Myc-HIS (zebrafish Vwf, (d–i)) plasmids were transfected into HEK293T cells. Anti-Myc antibody conjugated to Alexa Fluor 488 (green channel, (a, d, g)) was used for detection & anti-calnexin antibody conjugated to Alexa Fluor 594 (red channel, (b, e, h)) labeled endoplasmic reticulum (ER). Both constructs demonstrate formation of elongated Myc positive & ER negative structures (absence of yellow signal in the merged panels, (c, f, i)) characteristic of pseudo-WPBs (examples are indicated in (a, d), & (g) by arrowheads). Scale bars, 2.5 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23049555), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Calnexin Antibody - BSA Free [NB100-1974] - Liver-specific disruption of Chrebp in mice. A: Quantitative real-time PCR analysis of ChREBP mRNA. Total RNA was isolated from various tissues of control & liver-specific Chrebp knockout (L-Chrebp−/−) mice & subjected to real-time PCR analysis with 36B4 as the invariant control. Each value represents the mean ± SEM of four mice relative to that of controls, which was arbitrarily defined as 1.0. #P < 0.01 denotes the level of statistical significance (two-tailed Student’s t-test) between control & L-Chrebp−/− mice. B: Immunoblot analysis of ChREBP in liver lysates of control & L-Chrebp−/− mice. Aliquots (60 μg of protein) of liver whole-cell lysates were subjected to SDS-PAGE & immunoblot analysis with anti-ChREBP & anti-calnexin antibodies. ChREBP delta denotes a truncated aberrant ChREBP protein present only in lysates prepared from L-Chrebp−/− livers. The functional domains of ChREBP, including the glucose-sensing proline-rich bHLH-Zip DNA-binding & ZIP-like domains, are denoted. NLS, nuclear localization sequence. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0022227520331369), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Calnexin Antibody - BSA Free [NB100-1974] - Immunoblot analysis of livers from control & L-Chrebp−/− mice subjected to fasting & refeeding with a high-sucrose diet. Littermate control & L-Chrebp−/− mice (same as those described in supplemental Tables S2A & S2B) were subjected to fasting & refeeding. The nonfasted (N) groups were fed chow diet ad libitum. The fasted (F) group was fasted 12 h, & the refed (R) group was fasted for 12 h & then refed with 60% (w/w) high-sucrose diet for 12 h prior to study. Liver whole-cell lysates & membrane fractions were prepared individually, & equal amounts of protein from each mouse of the same group (four per group) were pooled. Aliquots (40 μg for whole-cell lysates & 30 μg for membrane fractions) of the pooled protein were subjected to SDS-PAGE & immunoblot analysis. Immunoblot analysis of Insig-1 & Insig-2 were carried out using membrane fractions. Whole-cell lysates were used to detect other proteins. The precursor & nuclear forms of SREBPs are denoted as P & N, respectively. Calnexin was used as loading control. Image collected & cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S0022227520331369), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Ha, Ze, Ca, Pm-Or

Species Glossary

Reactivity: Hu, Mu, Rt, Ha, Ze, Ca, Pm-Or

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-1974AF350
Alexa Fluor 405	NB100-1974AF405
Alexa Fluor 488	NB100-1974AF488
Alexa Fluor 532	NB100-1974AF532
Alexa Fluor 594	NB100-1974AF594
Alexa Fluor 647	NB100-1974AF647
Alexa Fluor 700	NB100-1974AF700
Alexa Fluor 750	NB100-1974AF750
Allophycocyanin	NB100-1974APC
Biotin	NB100-1974B
CoraFluor 1	NB100-1974CL1
DyLight 350	NB100-1974UV
DyLight 405	NB100-1974V
DyLight 488	NB100-1974G
DyLight 550	NB100-1974R
DyLight 594	NB100-1974DL594
DyLight 650	NB100-1974C
DyLight 680	NB100-1974FR
DyLight 755	NB100-1974IR
FITC	NB100-1974F
HRP	NB100-1974H
Janelia Fluor 525	NB100-1974JF525
Janelia Fluor 549	NB100-1974JF549
Janelia Fluor 585	NB100-1974JF585
Janelia Fluor 635	NB100-1974JF635
Janelia Fluor 646	NB100-1974JF646
Janelia Fluor 669	NB100-1974JF669
PE	NB100-1974PE
PerCP	NB100-1974PCP
mFluor Violet 450 SE	NB100-1974MFV450
mFluor Violet 500 SE	NB100-1974MFV500
mFluor Violet 610 SE	NB100-1974MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-1974-0.025ml

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NBP2-36571

Calnexin Antibody (IE2.1C12) - BSA Free

(2 Publications)

0.1 mg

NBP2-36571

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cells of Glomeruli developed strong cytoplasmic staining.

Flow Cytometry: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of Allophycocyanin conjugate of NBP2-36571. An intracellular stain was performed on Jurkat cells with Calnexin antibody (IE2.1C12) NBP2-36571APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining.

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin.

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration.

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein.

Flow (Intracellular): Calnexin Antibody (IE2.1C12) [NBP2-36571] - Intracellular staining of human Calnexin in Flow cytometry using 5.0 ug of antibody per 1 million cells. Isotype control was mouse IgG2b kappa.

Calnexin (IE2.1C12) was detected in immersion fixed Caco-2 human colorectal adenocarcinoma cell line using Mouse anti-Calnexin (IE2.1C12) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP2-36571AF488) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-36571AF350
Alexa Fluor 405	NBP2-36571AF405
Alexa Fluor 488	NBP2-36571AF488
Alexa Fluor 532	NBP2-36571AF532
Alexa Fluor 594	NBP2-36571AF594
Alexa Fluor 647	NBP2-36571AF647
Alexa Fluor 700	NBP2-36571AF700
Alexa Fluor 750	NBP2-36571AF750
Allophycocyanin	NBP2-36571APC
Biotin	NBP2-36571B
CoraFluor 1	NBP2-36571CL1
DyLight 350	NBP2-36571UV
DyLight 405	NBP2-36571V
DyLight 488	NBP2-36571G
DyLight 550	NBP2-36571R
DyLight 594	NBP2-36571DL594
DyLight 650	NBP2-36571C
DyLight 680	NBP2-36571FR
DyLight 755	NBP2-36571IR
FITC	NBP2-36571F
HRP	NBP2-36571H
Janelia Fluor 525	NBP2-36571JF525
Janelia Fluor 549	NBP2-36571JF549
Janelia Fluor 585	NBP2-36571JF585
Janelia Fluor 635	NBP2-36571JF635
Janelia Fluor 646	NBP2-36571JF646
Janelia Fluor 669	NBP2-36571JF669
PE	NBP2-36571PE
PerCP	NBP2-36571PCP
mFluor Violet 450 SE	NBP2-36571MFV450
mFluor Violet 500 SE	NBP2-36571MFV500
mFluor Violet 610 SE	NBP2-36571MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-36571SS
Azide and BSA Free	NBP2-80609

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NB300-518

Calnexin Antibody (AF18) - BSA Free

(14 Publications)

100 uL

NB300-518

Immunohistochemistry-Paraffin: Calnexin Antibody (AF18) [NB300-518] - Both normal and cancer biopsies of deparaffinized Human hepatocarcinoma tissues.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (AF18) [NB300-518] - Transfected COS7 cells were blocked in 1% BSA, 5% normal goat serum, and 0.1% Triton X-100 in 1X PBS, and then stained (1:500), followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody (red).

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (AF18) [NB300-518] - Staining of Calnexin in A549 Cells.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (AF18) [NB300-518] - Staining of HMVEC Cells.

Immunohistochemistry-Paraffin: Calnexin Antibody (AF18) [NB300-518] - Normal deparaffinized Human kidney tissue tissues.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-518AF350
Alexa Fluor 405	NB300-518AF405
Alexa Fluor 488	NB300-518AF488
Alexa Fluor 532	NB300-518AF532
Alexa Fluor 594	NB300-518AF594
Alexa Fluor 647	NB300-518AF647
Alexa Fluor 700	NB300-518AF700
Alexa Fluor 750	NB300-518AF750
Biotin	NB300-518B
CoraFluor 1	NB300-518CL1
DyLight 350	NB300-518UV
DyLight 405	NB300-518V
DyLight 488	NB300-518G
DyLight 550	NB300-518R
DyLight 594	NB300-518DL594
DyLight 650	NB300-518C
DyLight 680	NB300-518FR
DyLight 755	NB300-518IR
FITC	NB300-518F
HRP	NB300-518H
Janelia Fluor 525	NB300-518JF525
Janelia Fluor 549	NB300-518JF549
Janelia Fluor 585	NB300-518JF585
Janelia Fluor 635	NB300-518JF635
Janelia Fluor 646	NB300-518JF646
Janelia Fluor 669	NB300-518JF669
mFluor Violet 450 SE	NB300-518MFV450
mFluor Violet 500 SE	NB300-518MFV500
mFluor Violet 610 SE	NB300-518MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB300-518-0.025ml

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NBP1-97485

Calnexin Antibody

(12 Publications)

0.05 mg

NBP1-97485

Reactivity:

Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, Dr, Gp, Ha, Pm, Rb, Sh, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, Dr, Gp, Ha, Pm, Rb, Sh, Xp

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-36570

Calnexin Antibody (1C2.2D11) - BSA Free

(1 Publication)

0.1 mg

NBP2-36570

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cells of Glomeruli developed strong cytoplasmic staining.

Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin [1C2.2D11] Antibody NBP2-36570AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Calnexin (1C2.2D11) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein.

Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - Intracellular staining of human Calnexin in Flow cytometry using 5.0 ug of antibody per 1 million cells. Isotype control was mouse IgG2b kappa.

Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin Antibody (1C2.2D11) NBP2-36570F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Calnexin (IC2.2D11) was detected in immersion U-2 OS human osteosarcoma cell line using Mouse anti-Calnexin (IC2.2D11) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-36570AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

Reactivity:

Hu, Rt, Bv, Ca, Pm

Species Glossary

Reactivity: Hu, Rt, Bv, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-36570AF350
Alexa Fluor 405	NBP2-36570AF405
Alexa Fluor 488	NBP2-36570AF488
Alexa Fluor 532	NBP2-36570AF532
Alexa Fluor 594	NBP2-36570AF594
Alexa Fluor 647	NBP2-36570AF647
Alexa Fluor 700	NBP2-36570AF700
Alexa Fluor 750	NBP2-36570AF750
Allophycocyanin	NBP2-36570APC
Biotin	NBP2-36570B
CoraFluor 1	NBP2-36570CL1
DyLight 350	NBP2-36570UV
DyLight 405	NBP2-36570V
DyLight 488	NBP2-36570G
DyLight 550	NBP2-36570R
DyLight 594	NBP2-36570DL594
DyLight 650	NBP2-36570C
DyLight 680	NBP2-36570FR
DyLight 755	NBP2-36570IR
FITC	NBP2-36570F
HRP	NBP2-36570H
Janelia Fluor 525	NBP2-36570JF525
Janelia Fluor 549	NBP2-36570JF549
Janelia Fluor 585	NBP2-36570JF585
Janelia Fluor 635	NBP2-36570JF635
Janelia Fluor 646	NBP2-36570JF646
Janelia Fluor 669	NBP2-36570JF669
PE	NBP2-36570PE
PerCP	NBP2-36570PCP
mFluor Violet 450 SE	NBP2-36570MFV450
mFluor Violet 500 SE	NBP2-36570MFV500
mFluor Violet 610 SE	NBP2-36570MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-36570SS
Azide and BSA Free	NBP2-80608

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NBP2-43765

Calnexin Antibody (1563)

(5 Publications)

0.1 ml

NBP2-43765

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:500

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1563) [NBP2-43765] - Calnexin antibody [GT1563] detects Calnexin protein at cytoplasm by immunofluorescent analysis.Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calnexin stained by Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:500.Blue: Fluoroshield with DAPI .

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 50 ug mouse liver lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000

<b>Genetic Strategies Validation. </b>Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [1563] (NBP2-43765).

AKT acts down-stream of mTOR signaling. To examine if Akt is upstream or downstream of mTOR signaling (C) Cells cultured without pyruvate were exposed to Akt inhibitor (AI, 5 μM) or rapamycin (1, 10, or 100 nM), and mTOR phosphorylation, total mTOR levels and phosphorylation of p70S6K was assessed, calnexin was used as a protein loading control. The numbers above the blots indicate phospho-S6K levels relative to calnexin.

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (NBP2-43765) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-85519

Calnexin Antibody

(3 Publications)

0.1 ml

NBP1-85519

$Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.$

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex, placenta, testis and tonsil using Anti-Calnexin antibody NBP1-85519 (A) shows similar protein distribution across tissues to independent antibody NBP1-85520 (B).

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-47546

Calnexin Antibody (3H4A7)

(3 Publications)

0.1 ml

NBP1-47546

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-37774

Calnexin Antibody

(3 Publications)

0.1 mg

NBP1-37774

Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-37774] - Staining of paraffin embedded Human Testis. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Calnexin Antibody [NBP1-37774] - )Staining of Human Cerebellum (A) and Colorectal cancer (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Reactivity:

Hu, Mu, Rt, Po, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ca

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-30907

Alt Catalog# IMG-30907

Conjugate:

Unconjugated

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NBP1-97476

Calnexin Antibody

(3 Publications)

0.05 mg

NBP1-97476

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Gp, Ha, Pm, Rb, Sh, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ce, Ch, Gp, Ha, Pm, Rb, Sh, Xp

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-53352

Calnexin Antibody (CANX/1541)

(1 Publication)

100 μg

NBP2-53352

Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1541) [NBP2-53352] - Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).

Western Blot: Calnexin Antibody (CANX/1541) [NBP2-53352] - Western Blot Analysis of Human Kidney lysate using Calnexin Antibody (CANX/1541).

Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1541) [NBP2-53352] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).

Reactivity:

Hu, Rt(-)

Species Glossary

Reactivity: Hu, Rt(-)

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54552AF350
Alexa Fluor 405	NBP2-54552AF405
Alexa Fluor 488	NBP2-54552AF488
Alexa Fluor 532	NBP2-54552AF532
Alexa Fluor 594	NBP2-54552AF594
Alexa Fluor 647	NBP2-54552AF647
Alexa Fluor 700	NBP2-54552AF700
Alexa Fluor 750	NBP2-54552AF750
Biotin	NBP2-54552B
DyLight 350	NBP2-54552UV
DyLight 405	NBP2-54552V
DyLight 488	NBP2-54552G
DyLight 550	NBP2-54552R
DyLight 594	NBP2-54552DL594
DyLight 650	NBP2-54552C
DyLight 680	NBP2-54552FR
DyLight 755	NBP2-54552IR
FITC	NBP2-54552F
HRP	NBP2-54552H
Janelia Fluor 549	NBP2-54552JF549
Janelia Fluor 646	NBP2-54552JF646

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54552-100ug

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

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Species Glossary

Calnexin Antibodies

Calnexin Antibody - BSA Free

Calnexin Antibody - BSA Free

Calnexin Antibody (IE2.1C12) - BSA Free

Calnexin Antibody (AF18) - BSA Free

Calnexin Antibody

Calnexin Antibody (1C2.2D11) - BSA Free

Calnexin Antibody (1563)

Calnexin Antibody

Calnexin Antibody (3H4A7)

Calnexin Antibody

Calnexin Antibody

Calnexin Antibody (CANX/1541)