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APE Antibodies

We offer APE Antibodies for use in common research applications: Blocking/Neutralizing, Chromatin Immunoprecipitation, ELISA, Gel Super Shift Assays, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each APE Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our APE Antibodies can be used in a variety of model species: Human, Mouse, Primate, Rabbit, Rat. Use the list below to choose the APE Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our APE polyclonal antibodies and browse our APE monoclonal antibody catalog.

Alternate Names for APE Antibodies

anti-APE antibody, anti-APEX1 antibody, anti-AP endonuclease 1 antibody, anti-APE-1 antibody, anti-APE1 antibody, anti-APEAPEX nuclease (multifunctional DNA repair enzyme) antibody, anti-APENAP endonuclease class I antibody, anti-APEX deoxyribonuclease (apurinic or apyrimidinic) antibody, anti-APEX nuclease (multifunctional DNA repair enzyme) 1 antibody, anti-APEX nuclease antibody, anti-Apurinic-apyrimidinic endonuclease 1 antibody, anti-APXAP lyase antibody, anti-DNA-(apurinic or apyrimidinic site) lyase antibody, anti-EC 3.1 antibody, anti-EC 4.2.99.18 antibody, anti-HAP1apurinic/apyrimidinic (abasic) endonuclease antibody, anti-multifunctional DNA repair enzyme antibody, anti-protein REF-1 antibody, anti-redox factor 1 antibody, anti-Redox factor-1 antibody, anti-REF-1 antibody, anti-REF1 apurinic/apyrimidinic exonuclease antibody

11 Results for "ape" in Primary Antibodies

NB100-116

APE Antibody (13B8E5C2) - BSA Free

(3 Reviews)

(105 Publications)

0.1 mg

NB100-116

Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

<b>Genetic Strategies Validation. </b>Knockdown Validated: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1, p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - HeLa cells stained NB100-116 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).

Immunohistochemistry: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Immunohistochemical staining of APE1. A. Nuclear staining in the luminal epithelium of normal breast ducts and lobules. B. Low, and C. high nuclear APE1 expression in invasive breast cancer. (magnification x400). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0099528) licensed under a CC-BY license.

Chromatin Immunoprecipitation (ChIP): APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Association of p53 and APE1 on p53-binding sites in p21 promoter. Re-ChIP analysis (first IP with alpha-APE1 and the second IP with alpha-p53 antibody) showing simultaneous recruitment of APE1 and p53 in control vs. EPE treated cells; *: p value 12 in each group. (B) Western blot images using S100 beta , tubulin beta‐3, and beta ‐actin antibodies to total protein lysate in hippocampal area. The statistical results of internal standardized S100 beta / beta ‐tubulin ratio by beta ‐actin levels relative to young adult wild‐type C57BL/6J. White and black bars indicate the wild‐type C57BL/6J and Tet‐mev‐1 mice, respectively. Data are expressed as mean ± SD; *P 12 in each group. (C) Micrographs of immunohistochemical analysis on paraffined hippocampal tissue sections using GFAP and S100 beta antibody. Brown cells indicate GFAP‐stained astrocytes. Scale bar = 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27623715), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

EBNA1 promotes the upregulation of oxidative DNA damage repair pathways in EBV converted cell lines and EBV positive BLs. a Representative western blots illustrating the expression of MTH1, OGG1, and MUTYH in pairs of EBV-negative and -positive cell lines. GAPDH was used as loading control. b Densitometry quantification of the specific bands. The intensity of the specific band in EBV positive cells relative the EBV-negative parental is shown. Mean ± SE of four independent experiments. c Representative western blots illustrating the expression of EBNA1, MTH1, OGG1, and MUTYH in the Mutu cell lines. d Densitometry quantification of expression in the EBV positive cell lines relative to the EBV-negative Mutu-30. Mean ± SE of four independent experiments Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31511648), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western blot images (A) and quantifications (B–G) of cell lysates from C2C12 myotubes with control or UCHL1 siRNA knockdown for perilipin 2 (B), perilipin 5 (C), CD36 (D), HSL (E), MAGL (F), and SDHB (G). n = 4 per group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35464088), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

BDNF lowered the mechanical withdrawal threshold further and promoted activation of astrocytes and microglia, and enhanced the p38/JNK pathway to aggravate the release of IL-1 beta and TNF-alpha in the SDH of CYP-induced cystitis. a BDNF treated every other day after CYP injection could further lower the mechanical withdrawal threshold and suppress the retrieval of mechanical threshold when compared with the CYP + PBS group. After the exogenous BDNF injection, Western blots showing the expression of b BDNF, c TrkB, dp-TrkB, e Iba1, f GFAP, gp-p38, hp-JNK, i TNF-alpha , and j IL-1 beta were all further upregulated when compared with the CYP + PBS group. Data of mechanical withdrawal threshold were analyzed using a two-way analysis of variance (ANOVA) followed by the Sidak's multiple comparisons test. All data were calculated as mean ± SEM (n = 10 per group). **p < 0.01, ***p < 0.001 vs. the control group. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. the CYP + rBDNF group. $p < 0.05, $$p < 0.01, $$$p < 0.001 vs. the CYP + PBS group Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31931832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Primary human grade IV glioblastoma xenotransplant.(A) Dynamic PET image (coronal) of advanced tumor in the right hemisphere of the brain with [18F]PBR06 uptake confined primarily to the tumor. Arrows indicate tumor and infiltration into left hemisphere. (B) Time-activity curves of injected [18F]PBR06 in tumor (green) and contralateral brain (blue) [30]. (C) Correlative TSPO immunohistochemistry, Tumor + White Matter Tract (40X). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0141659), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , , , , , , , ,

Applications: , , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-116AF350
Alexa Fluor 405	NB100-116AF405
Alexa Fluor 488	NB100-116AF488
Alexa Fluor 532	NB100-116AF532
Alexa Fluor 594	NB100-116AF594
Alexa Fluor 647	NB100-116AF647
Alexa Fluor 700	NB100-116AF700
Alexa Fluor 750	NB100-116AF750
Allophycocyanin	NB100-116APC
Biotin	NB100-116B
DyLight 350	NB100-116UV
DyLight 405	NB100-116V
DyLight 488	NB100-116G
DyLight 550	NB100-116R
DyLight 594	NB100-116DL594
DyLight 650	NB100-116C
DyLight 680	NB100-116FR
DyLight 755	NB100-116IR
FITC	NB100-116F
HRP	NB100-116H
Janelia Fluor 549	NB100-116JF549
Janelia Fluor 646	NB100-116JF646
PE	NB100-116PE
PerCP	NB100-116PCP
mFluor Violet 450 SE	NB100-116MFV450
mFluor Violet 500 SE	NB100-116MFV500
mFluor Violet 610 SE	NB100-116MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-116SS
Azide and BSA Free	NBP2-80578

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NB100-101

APE Antibody - BSA Free

(3 Reviews)

(70 Publications)

0.1 ml

NB100-101

Simple Western: APE Antibody [NB100-101] - Lane view shows a specific band for APE1 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: APE Antibody [NB100-101] - Analysis of APE1 in cell lysates: 1. HeLa, 2. Ntera2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH 3T3, 7. PC12, and 8. Cos 7.

Immunohistochemistry: APE Antibody [NB100-101] - Immunohistochemical staining of APE-ref-1 in prostate cancer.

Western Blot: APE Antibody [NB100-101] - RKO cells were subjected to scrambled siRNA (Mock) or siRNA against TDP1 (TDP1KD) and cell lysates analyzed by immunoblotting (left). Control RKO (RKOSc siRNA) and RKO cells in which TDP1 levels were depleted (RKOTDP1 siRNA) were examined for their survival following exposure to the indicated doses of MMS, as described earlier in the text (right). Increasing the load of unrepaired methylated purines by exploiting the limited availability of TDP1 alone or in combination with canonical BER factors such as APE1 provides a new synthetic lethal setting to improve the clinical outcome of temozolomide-based cancer therapy. Image collected and cropped by CiteAb from the following publication (academic.oup.com/nar/article/42/5/3089/1051107), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: APE Antibody [NB100-101] - IF on HeLa cells. Image from verified customer review.

Western Blot: APE Antibody [NB100-101] - SH-SY5Y (nuclear extract) tested at 1:1000 dilution. Image provided by verified customer review.

Flow Cytometry: APE Antibody [NB100-101] - Spectral properties of Alexa Fluor(R) 488: an excitation maximum of 490 nm and an emission maximum of 525 nm. Use the Novus Spectra Viewer to identify the optimal laser(s) and filters for Alexa Fluor(R) 488 and to determine its compatibility with other fluorophores when designing a multicolor experiment. //www.novusbio.com/spectraviewer

Western Blot: APE Antibody [NB100-101] - Analysis of APE in human melanoma cell lysate using anti-APE antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: APE Antibody [NB100-101] - Detection of APE1 (Green) in HepG2 cells using NB100-101. Nuclei (Blue) are counterstained with Hoechst 33258.

APE1 expression in HCC tissues. (C) Western blot analysis of HCC and SLC tissue lysates patients performed on pooled samples from HCC cancer. Actin was used as loading control and for the relative normalization. A representative image of western blot analysis is shown. Data represent the means of +/- SD of three independent experiments. *P < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30719231), licensed under a CC-BY licence.

Aphidicolin-induced ATM signaling in immortalized MEFs. (A) Cells (WT, MJI-53; KO, MJI-1) were treated for 24 h using 0 or 3 μM aphidicolin. (B) Cells (lane 1–3, WT, MJI-91; lanes 4–6, KO, MJI-186; lanes 7–9, WT, MJI-105; lanes 1–12, KO, MJI-84) were treated for 24 h using 0, 1 or 3 μM aphidicolin. Actin and 53BP1 serve as loading controls. DYNLL1 is used as a surrogate marker for loss of ASCIZ. (C) Quantification of western blot band intensities. An arbitrary unit of 100 represents the average band intensity for the respective phospho-protein in the 3 μM aphidicolin-treated wildtype samples on each membrane. Graphs indicate the mean ± standard error, n = 3. Additional loading controls for total KAP1, p53 and H2AX are shown in Supplementary Fig. S1A and B. Image collected and cropped by CiteAb from the following open publication (//linkinghub.elsevier.com/retrieve/pii/S1568786417301349), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Autophagy responds rapidly to changing glucose conditions. Immortalized MSCs were cultured in physiologic (also called low in culture parlance; 1 g/L; 5.5 mM) or high (4.5 g/L; 25 mM) glucose media for 2 days and then changed to the corresponding opposite glucose concentration for up to 96 h. Myosin light chain 3 (LC3) levels were probed via immunoblot to assess autophagic response (a). The role of oxygen in the glucose response was also assessed by culturing the MSCs in a Biospherix hypoxic chamber at 4% and 1% oxygen in high and low glucose media for 4 days, followed by comparable LC3 blots (b). Shown are representative blots of three repeated studies. alpha -Actinin was used as a housekeeping control for all blots Image collected and cropped by CiteAb from the following open publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-016-0436-7), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Chromatin opening activates DDR via a mechanism that requires catalytically functional LOXL2 but can be independent of DNA damage. a gamma -H2AX and 53BP1 staining and foci quantification are shown by immunofluorescence with a specific antibody for gamma -H2AX (left image) or for 53BP1 (right image). Dot graphs indicate the number of foci for gamma -H2AX (upper graph) and 53BP1 (lower graph) per cell in control and LOXL2 KD conditions. b gamma -H2AX staining and foci quantification are shown by immunofluorescence with the indicated antibody after LOXL2 reinfection. MDA-MB-231 cells were first infected with control or LOXL2 KD lentivirus and then, after puromycin selection, again with GFP (MOCKGFP+), wild-type LOXL2-IRES-GFP (LOXL2GFP+), or LOXL2mut-IRES-GFP (LOXL2mutGFP+). Cells were fixed after 24 h. Dot graphs indicate the number of gamma -H2AX foci per GFP-positive cells containing MOCKGFP+ (upper graph), LOXL2GFP+ (middle graph), or LOXL2mutGFP+ (lower graph). c Dot graphs indicate the number of gamma -H2AX (upper graph) and 53BP1 (lower graph) foci per cell in control and LOXL2 KD cells after treatment with 200 μM cordycepin for the indicated timepoints. d gamma -H2AX and 53BP1 staining and foci quantification are shown after immunofluorescence with the indicated antibodies in non-replicative conditions. Dot graphs indicate the number of the gamma -H2AX (left graph) and 53BP1 (right graph) foci in control and LOXL2 KD cells. e Representative image showing DNA damage in control or LOXL2 KD MDA-MB-231 cells, visualized by the alkaline comet assay. Cells were treated with 0.3 μM doxorubicin for 24 h. The graph shows the percentage of MDA-MB-231 positive cells. f Chromosome alterations in control and LOXL2 KD MDA-MB-231 cells. Error bars indicate the SD from at least three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001, ****P < 0.0001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31462706), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Rb

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-101AF350
Alexa Fluor 405	NB100-101AF405
Alexa Fluor 488	NB100-101AF488
Alexa Fluor 532	NB100-101AF532
Alexa Fluor 594	NB100-101AF594
Alexa Fluor 647	NB100-101AF647
Alexa Fluor 700	NB100-101AF700
Alexa Fluor 750	NB100-101AF750
Allophycocyanin	NB100-101APC
Biotin	NB100-101B
DyLight 350	NB100-101UV
DyLight 405	NB100-101V
DyLight 488	NB100-101G
DyLight 550	NB100-101R
DyLight 594	NB100-101DL594
DyLight 650	NB100-101C
DyLight 680	NB100-101FR
DyLight 755	NB100-101IR
FITC	NB100-101F
HRP	NB100-101H
PE	NB100-101PE
PerCP	NB100-101PCP
mFluor Violet 450 SE	NB100-101MFV450
mFluor Violet 500 SE	NB100-101MFV500
mFluor Violet 610 SE	NB100-101MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-101SS

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AF1044

APE Antibody [Unconjugated]

(2 Publications)

100 μg

AF1044

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat APE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1044) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for APE at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for APE at approximately 45 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat APE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1044) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

APE was detected in immersion fixed A549 human lung carcinoma cells using Goat Anti-Human/Mouse/Rat APE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1044) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Nuclear. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

APE was detected in immersion fixed HepG2 human hepatocellular carcinoma cells using Goat Anti-Human/Mouse/Rat APE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1044) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Nuclear. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1044-SP

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NB100-897

APE Antibody

(6 Publications)

0.1 mg

NB100-897

Western Blot: APE Antibody [NB100-897] - Staining of A431 (A), HeLa (B) and MCF7 (C) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: APE Antibody [NB100-897] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Reactivity:

Hu, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Po, Bv, Ca

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP2-54653

APE Antibody

100 ul

NBP2-54653

Western Blot: APE Antibody [NBP2-54653] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human cell line A-431 Lane 5: Human liver tissue Lane 6: Human tonsil tissue

Immunocytochemistry/Immunofluorescence: APE Antibody [NBP2-54653] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining of human skeletal muscle shows low expression as expected.

Western Blot: APE Antibody [NBP2-54653] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining of human small intestine shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining of human endometrium shows high expression.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: APE Antibody [NBP2-54653] - Staining in human endometrium and skeletal muscle tissues using anti-APEX1 antibody. Corresponding APEX1 RNA-seq data are presented for the same tissues.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-07864

APE Antibody (CPTC-APEX1-2)

100 μg

NBP3-07864

Immunocytochemistry/Immunofluorescence: APE Antibody (CPTC-APEX1-2) [NBP3-07864] - Immunofluorescence Analysis of human HeLa cells labeling APE with APE Mouse Monoclonal Antibody (CPTC-APEX1-2) followed by goat anti- Mouse IgG-CF488 (Green). Phalloidin CF640 stains the membrane red.

Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) [NBP3-07864] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APE Mouse Monoclonal Antibody (CPTC-APEX1-2).

Flow Cytometry: APE Antibody (CPTC-APEX1-2) [NBP3-07864] - Flow Cytometric Analysis of HeLa cells using APE Mouse Monoclonal Antibody (CPTC-APEX1-2). Goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: APE Antibody (CPTC-APEX1-2) [NBP3-07864] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with APE Mouse Monoclonal Antibody (CPTC-APEX1-2).

Protein Array: APE Antibody (CPTC-APEX1-2) [NBP3-07864] - Analysis of Protein Array containing more than 19,000 full-length human proteins using APE Mouse Monoclonal Antibody (CPTC-APEX1-2).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-08370AF350
Alexa Fluor 405	NBP3-08370AF405
Alexa Fluor 488	NBP3-08370AF488
Alexa Fluor 532	NBP3-08370AF532
Alexa Fluor 594	NBP3-08370AF594
Alexa Fluor 647	NBP3-08370AF647
Alexa Fluor 700	NBP3-08370AF700
Alexa Fluor 750	NBP3-08370AF750
Allophycocyanin	NBP3-08370APC
Biotin	NBP3-08370B
DyLight 350	NBP3-08370UV
DyLight 405	NBP3-08370V
DyLight 488	NBP3-08370G
DyLight 550	NBP3-08370R
DyLight 594	NBP3-08370DL594
DyLight 650	NBP3-08370C
DyLight 680	NBP3-08370FR
DyLight 755	NBP3-08370IR
FITC	NBP3-08370F
HRP	NBP3-08370H
Janelia Fluor 549	NBP3-08370JF549
Janelia Fluor 646	NBP3-08370JF646
PE	NBP3-08370PE
PerCP	NBP3-08370PCP
mFluor Violet 450 SE	NBP3-08370MFV450
mFluor Violet 500 SE	NBP3-08370MFV500
mFluor Violet 610 SE	NBP3-08370MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-08370

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NB100-56359

APE Antibody

0.1 mg

NB100-56359

Western Blot: APE Antibody [NB100-56359] - Analysis of APE in HeLa lysates using APE antibody at 2 ug/mL.

Immunohistochemistry-Paraffin: APE Antibody [NB100-56359] - Human Breast: FFPE tissue section using APE antibody at 10 ug/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-392

Alt Catalog# IMG-392

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB1044

APE Antibody (200913)

(1 Publication)

100 μg

MAB1044

Western blot shows lysates of NRK rat normal kidney cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat APE Monoclonal Antibody (Catalog # MAB1044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APE at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1044-SP

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NBP3-15361

APE Antibody (3W5N10)

100 ul

NBP3-15361

Western Blot: APE Antibody (3W5N10) [NBP3-15361] - Western blot analysis of extracts of various cell lines, using APEX1/APE Rabbit mAb (NBP3-15361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: APE Antibody (3W5N10) [NBP3-15361] - Immunohistochemistry of paraffin-embedded human colon using APEX1/APE Rabbit mAb (NBP3-15361) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-15113

APE Antibody (S07-5A9)

100 ul

NBP3-15113

Western Blot: APE Antibody (S07-5A9) [NBP3-15113] - Western blot detection of APE in K562, C6, 3T3, Hela cell lysates using APE Rabbit mAb (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 36kDa.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-76495

APE Antibody

100 ul

NBP2-76495

Immunocytochemistry/Immunofluorescence: APE Antibody [NBP2-76495] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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(select up to 3 products from this page to compare)

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