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Microtubule Polymerization Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Microtubule Polymerization Pathway and Malignant Neoplasms, Neoplasms, Malignant Neoplasm Of Breast, Mammary Neoplasms, Carcinoma. The study of the Microtubule Polymerization Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Microtubule Polymerization Pathway has been researched in relation to Mitosis, Cell Cycle, Transport, Microtubule Depolymerization, Interphase. The Microtubule Polymerization Pathway complements our catalog of research reagents including antibodies and ELISA kits against APC, BCL2, CASP3, CCNB1, CDK1.

Top Research Reagents

We have 3976 products for the study of the Microtubule Polymerization Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NBP3-17099
Immunohistochemistry-Paraffin: ANKS1B Antibody [NBP3-17099] - Staining of human cerebral cortex shows high expression.Immunohistochemistry-Paraffin: ANKS1B Antibody [NBP3-17099] - Analysis in human cerebral cortex and liver tissues using Anti-ANKS1B antibody. Corresponding ANKS1B RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NB500-181
Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eg5.Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NB500-181] - Staining of HeLa cells fixed in 3.5% paraformaldehyde.  Cytoplasmic staining during interphase and spindle staining during mitosis.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IB, ICC/IF

     2 Reviews

21 Publications
NB300-213
Mouse primary cultures stained with MAP2 antibody. ICC/IF image submitted by a verified customer review.Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to microtubule associated protein 2 (MAP2), NB300-213, dilution 1:10,000 in red, and mouse mAb to MAP-tau, NPB2-25162, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. NB300-213 antibody stains dendrites and perikarya of neurons, while NBP2-25162 antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.

Chicken Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     5 Reviews

115 Publications
H00009167-B02P
Western Blot: COX7A2L Antibody [H00009167-B02P] - Analysis of COX7A2L expression in HepG2.Western Blot: COX7A2L Antibody [H00009167-B02P] - analysis of COX7A2L expression in transfected 293T cell line by COX7A2L MaxPab polyclonal antibody. Lane 1: COX7A2L transfected lysate (12.54 KDa). Lane 2: Non-transfected lysate.

Mouse Polyclonal
Species Human
Applications WB

NBP1-48291
Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug ofImmunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded human endometrium tissue using PLK1 antibody (OTI1D4). Primary antibody dilution 1:200. Image from verified customer review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IHC

     1 Review

NBP1-72042
Western Blot: Doublecortin Antibody [NBP1-72042] - staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

4 Publications
NBP1-76798
Knockout Validated: Stathmin 1 Antibody [NBP1-76798] -  Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Stathmin 1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-76798) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Stathmin 1 at approximately 17 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Western Blot: Stathmin 1 Antibody [NBP1-76798] - EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

NBP1-84928
Western Blot: MAPRE1 Antibody [NBP1-84928] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.Immunocytochemistry/Immunofluorescence: MAPRE1 Antibody [NBP1-84928] - Staining of human cell line U-251 MG shows localization to cytosol and microtubule organizing center. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-85729
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

5 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     3 Reviews

42 Publications
NBP2-00714
Western Blot: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EB2.Immunocytochemistry/Immunofluorescence: EB2 Antibody (1F3) [NBP2-00714] Staining of COS7 cells transiently transfected by pCMV6-ENTRY EB2.

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

1 Publication
NB100-56098
Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.Western Blot: Bcl-2 Antibody [NB100-56098] - EA reduced MPP+-induced dopaminergic neuronal apoptosis by increasing BDNF (brain-derived neurotrophic factor) expression and further Akt phosphorylation in the rat substantia nigra. Eight days after MPP+ administration, our Western blot results (MAB7566) show that MPP+ treatment reduced tyrosine hydroxylase and Bcl-2 expression in the ipsilateral side of the rat substantia nigra (SN), but not in the contralateral side. EA stimulation (50 Hz) enhanced mature BDNF, tyrosine hydroxylase, and Bcl-2 expression in the MPP+-treated ipsilateral side. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/18/9/1846), licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

     2 Reviews

26 Publications
NBP2-25162
Western Blot: Tau Antibody (2E9) [NBP2-25162] - Analysis of different tissue lysates using mouse mAb to MAP-tau, NBP2-25162, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weight, and so appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS and including larger big tau forms in the PNS, visible in lane 5.Immunocytochemistry/Immunofluorescence: Tau Antibody (2E9) [NBP2-25162] - Analysis of cortical neuron-glial culture from E20 rat stained with mouse mAb to MAP-tau, NBP2-25162, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:5,000 in red. The blue is DAPI staining of nuclear DNA. NBP2-25162 antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both proteins.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

5 Publications
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).

Mouse Monoclonal
Species Human, Mouse, Chimpanzee
Applications WB, Flow, ICC/IF

24 Publications
AF1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=    ERK2  was detected in immersion fixed paraffin-embedded sections of human breast  using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal  Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at room  temperature followed by incubation with the Anti-Rabbit IgG  VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, KO

3 Publications
AF6000
Western blot shows lysates of U2OS human osteosarcoma cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, IHC

12 Publications
AF835
Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for <a class=Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # <a class=

Rabbit Polyclonal
Species Human, Mouse
Applications IHC, ICC

     7 Reviews

386 Publications
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate.  Lane2:HEK293T cell lysate.  Lane3:Rat testis tissue lysate.  Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

2 Publications
NBP3-05513
Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-METAP2 antibody. Remaining relative intensity is presented.Immunocytochemistry/Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC