Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eg5.](http://images.novusbio.com/fullsize/Eg5-Antibody-Western-Blot-NB500-181-img0005.jpg "Western Blot: Eg5 Antibody [NB500-181] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Eg5.")
![Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NB500-181] - Staining of HeLa cells fixed in 3.5% paraformaldehyde. Cytoplasmic staining during interphase and spindle staining during mitosis.](http://images.novusbio.com/fullsize/Eg5-Antibody-Immunocytochemistry-Immunofluorescence-NB500-181-img0003.jpg)
![Western Blot: Eg5 Antibody [NB500-181] - Human Eg5 rescues depletion of the Drosophila kinesin-5 family member Klp61F. Western blot analysis of cell lysates prepared from induced and uninduced; control and Klp61F RNAi-treated cells blotted with anti-Eg5 to monitor induction of Eg5-mCherry, anti-Klp61F to confirm depletion of the motor and anti-alpha-tubulin as a loading control. Cells depleted of Klp61F and with Eg5-mCherry induced are referred to as RepEg5 cells. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fonc.2013.00187/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Eg5-Antibody-Western-Blot-NB500-181-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Eg5 Antibody [NB500-181] - Spontaneous assembly of rosettes in iChM5 derivatives.Feeder free cultures iChM5Ap15 & iChM5Bp28 cells in chamber slides were immunostained as indicated. Left panel, emerging rosettes among self-renewing iPSCs; grayscale inset shows 1x magnification of immunostaining of Oct4 alone. Middle panel shows Nanog staining alone with inset at 2X magnification showing presumptive centrosomes (arrows). Bottom left panel shows forming rosettes immunopositive for Sox2 & Eg5. Bottom right panel shows low magnification image of immunostaining of Sox2 in this iChM5Ap15 culture, showing that virtually all cells were immunopositive. Grey scale inset shows representative forming rosette. Asterisks (*) in each panel indicates example of forming rosette. Scale bar, 50 microns. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25426336), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-181_rabbit-polyclonal-eg5-antibody-310202416171491.jpg)
![Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Analysis in human tonsil and pancreas tissues. Corresponding KIF11 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Eg5-Antibody-Immunohistochemistry-Paraffin-NBP1-84887-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Analysis in human tonsil and pancreas tissues. Corresponding KIF11 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Eg5 Antibody [NBP1-84887] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Eg5-Antibody-Western-Blot-NBP1-84887-img0008.jpg)
![Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human tonsil shows moderate to strong cytoplasmic positivity, mainly in germinal center cells.](http://images.novusbio.com/fullsize/Eg5-Antibody-Immunohistochemistry-Paraffin-NBP1-84887-img0015.jpg)
![Western Blot: Eg5 Antibody [NBP1-84887] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/Eg5-Antibody-Western-Blot-NBP1-84887-img0007.jpg)
![Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Eg5-Antibody-Immunohistochemistry-Paraffin-NBP1-84887-img0013.jpg)
![Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Eg5-Antibody-Immunohistochemistry-Paraffin-NBP1-84887-img0014.jpg)
![Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Eg5-Antibody-Immunohistochemistry-Paraffin-NBP1-84887-img0016.jpg)
![Western Blot: Eg5 Antibody [NBP1-84888] - Analysis in control (vector only transfected HEK293T lysate) and KIF11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Eg5-Antibody-Western-Blot-NBP1-84888-img0007.jpg "Western Blot: Eg5 Antibody [NBP1-84888] - Analysis in control (vector only transfected HEK293T lysate) and KIF11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84888] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Eg5-Antibody-Immunohistochemistry-Paraffin-NBP1-84888-img0006.jpg)
![Western Blot: Eg5 Antibody [NBP2-58929] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/Eg5-Antibody-Western-Blot-NBP2-58929-img0002.jpg "Western Blot: Eg5 Antibody [NBP2-58929] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: Eg5 Antibody [NBP2-58929] - Staining of human cell line RH-30 shows localization to cytosol.](http://images.novusbio.com/fullsize/Eg5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58929-img0001.jpg)
![SDS-Page: Recombinant Human Eg5 Protein [H00003832-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00003832-Q01-1.jpg "SDS-Page: Recombinant Human Eg5 Protein [H00003832-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Eg5 Antibody [NB100-57514] - Detection of Human EG5/KIF11 on HeLa whole cell lysate using NB100-57514. EG5/KIF11 was also immunoprecipitated by rabbit anti-EG5/KIF11 antibody NB100-57513.](http://images.novusbio.com/fullsize/Eg5-Antibody-Western-Blot-NB100-57514-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Eg5 Antibody [NB100-57514] - Detection of Human EG5/KIF11 on HeLa whole cell lysate using NB100-57514. EG5/KIF11 was also immunoprecipitated by rabbit anti-EG5/KIF11 antibody NB100-57513.")
![Western Blot: Eg5 Overexpression Lysate (Adult Normal) [NBL1-12284] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Eg5.](http://images.novusbio.com/fullsize/Eg5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12284-img0002.jpg "Western Blot: Eg5 Overexpression Lysate (Adult Normal) [NBL1-12284] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Eg5.")
![Western Blot: Eg5 Antibody (6G8V8) [NBP3-16458] - Western blot analysis of extracts of various cell lines, using Eg5 Rabbit mAb (NBP3-16458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Eg5-Antibody-6G8V8-Western-Blot-NBP3-16458-img0001.jpg "Western Blot: Eg5 Antibody (6G8V8) [NBP3-16458] - Western blot analysis of extracts of various cell lines, using Eg5 Rabbit mAb (NBP3-16458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Eg5 Antibody (1G11) [NBP3-26182] - Positive Western Blot detected in: MCF-7 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, U-251 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: Eg5 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120 kDa Observed band size: 130 kDa](http://images.novusbio.com/fullsize/nbp3-26182_rabbit-eg5-mab-1g11-28220241140930.jpg "Western Blot: Eg5 Antibody (1G11) [NBP3-26182] - Positive Western Blot detected in: MCF-7 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate, U-251 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: Eg5 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120 kDa Observed band size: 130 kDa")